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Th1-polarized cytokine production by human B cells


Gagro, Alenka; Servis, Dražen; Toellner, K.M.; Grafton, G.; Taylor, D.R.; Branica, Srećko; Gordon, John
Th1-polarized cytokine production by human B cells // Abstracts
Groningen, Nizozemska, 2002. str. 33-33 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)


Naslov
Th1-polarized cytokine production by human B cells

Autori
Gagro, Alenka ; Servis, Dražen ; Toellner, K.M. ; Grafton, G. ; Taylor, D.R. ; Branica, Srećko ; Gordon, John

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts / - , 2002, 33-33

Skup
14th Germinal Centre Conference

Mjesto i datum
Groningen, Nizozemska, 22-28.06.2002

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
B cells

Sažetak
B cells, in their resting state, lack antigen presenting capacity, but shortly after crosslinking of BCR and/or ligation of CD40, they upregulate B7 molecules and differentiate into efficient APC. The recent finding that murine na&iuml ; ve B cells can differentiate into effector subsets 1 and 2, which produce Th1- and Th2-polarising cytokines, respectively, has suggested that B cells have the capacity to amplify or maintain an ongoing polarised immune response. However, the activation requirements of naive vs memory B cells for cytokine production could be different. Human na&iuml ; ve and memory B cells can be readily distinguished by the expression of CD27, a molecule that is absent on na&iuml ; ve B cells. Here we compared human na&iuml ; ve (IgM+IgD+CD27-CD38-IgG-IgA-) and memory (IgM-IgD-CD27+CD38-IgG+/-IgA+/-) tonsillar B cells for their expression of IL-12 and IL-23 and the signals that induce these cytokines. We found that B cell subsets when engaged in signaling via BCR and CD40 do not modulate p35 subunit of IL-12 as measured by quantitative PCR. CD40 ligation induced p40 IL-12 measured both by quantitative PCR and p40 ELISA significantly more in memory B cells. However, neither B cell subsets secreted significant amounts of p70 IL-12. Memory B cells also expressed more p19 IL-23 mRNA, but the major signal responsible for upregulation of this cytokine was BCR trigerring. The inability of B cells to produce the p70 IL-12 heterodimer would limit their capacity as frontline APC for the initiation of Th1 responses. However, the ability of memory B cells to produce IL-23 offers this B cell subset the capacity to maintain and amplify Th1-direction of skewing following subsequent encounters with the antigen.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Projekt / tema
0021004

Ustanove
Imunološki zavod d.d.

Profili:

Avatar Url Alenka Gagro (autor)

Avatar Url Srećko Branica (autor)

Avatar Url Dražen Servis (autor)

Citiraj ovu publikaciju

Gagro, Alenka; Servis, Dražen; Toellner, K.M.; Grafton, G.; Taylor, D.R.; Branica, Srećko; Gordon, John
Th1-polarized cytokine production by human B cells // Abstracts
Groningen, Nizozemska, 2002. str. 33-33 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
Gagro, A., Servis, D., Toellner, K., Grafton, G., Taylor, D., Branica, S. & Gordon, J. (2002) Th1-polarized cytokine production by human B cells. U: Abstracts.
@article{article, year = {2002}, pages = {33-33}, keywords = {B cells}, title = {Th1-polarized cytokine production by human B cells}, keyword = {B cells}, publisherplace = {Groningen, Nizozemska} }