engleski

Production, purification and characterization of lipase from Thermomyces lanuginosus

The aim of the research was to determinate optimal process conditions for Thermomyces lanuginosus lipase (TLL) production during solid-state cultivation on hull-less pumpkin oil pomace. Numerical optimization of the five independent variables (substrate mass (ms), inoculum size (n), moisture content (wH2O), fermentation time (t) and temperature (T)) which affected the TLL production was evaluated using response surface methodology. To predict the TLL production, reduced quadratic mathematical model was proposed and model validation was performed under optimized conditions (ms = 50 g, n = 7, wH2O = 60%, t = 2 days, T = 45 °C) with 95% accuracy. The simplified purification method of the TLL was developed which includes a single-step vacuum-evaporation technique. Produced TLL liquid preparation with high volumetric activity (422 U cm-3) was obeyed. TLL was characterized as thermostable biocatalyst which possesses stability in a variety of organic solvents, food oils and fats. The sustainability of the process was confirmed by the green chemistry matrix of the E-factor and process mass index.

Thermomyces lanuginosus ; lipase ; enzyme purification ; sustainability

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