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Pregled bibliografske jedinice broj: 1080365

Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA


Ptiček, Lucija; Racane, Livio; Zonjić, Iva; Tumir, Lidija-Marija; Radić Stojković, Marijana
Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA // Book of abstracts, 18th Ružička Days: Today science – tomorrow industry / Jukić, Ante (ur.).
Zagreb: HDKI, 2020. str. 46-46 (poster, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 1080365 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA

Autori
Ptiček, Lucija ; Racane, Livio ; Zonjić, Iva ; Tumir, Lidija-Marija ; Radić Stojković, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts, 18th Ružička Days: Today science – tomorrow industry / Jukić, Ante - Zagreb : HDKI, 2020, 46-46

ISBN
978-953-6894-75-8

Skup
18th Ružička Days: Today science – tomorrow industry

Mjesto i datum
Vukovar, Hrvatska, 16-18.09.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
amidines ; benzazoles ; interactions ; DNA/RNA

Sažetak
Series of eleven novel amidino-substituted bis(benzazoles), differing in heteroaromatic scaffolds as well as in type and position of amidine moiety, were studied for their binding abilities for DNA and RNA. By introducing different types of amidinic substituents and heteroaromatic substructure, namely benzothiazole, benzimidazole and benzoxazole, presented compounds were synthesized by condensation reaction of amidino-substituted 2- aminothiophenols, 2-aminophenols or o- phenylenediamines with isomeric aromatic dialdehydes or dicarboxylic acids by our previously developed method [1, 2]. Noncovalent binding interactions with DNA and RNA like intercalation, groove and electrostatic binding can be investigated by variety of methods. In order to detect possible presence of DNA/RNA active compounds in extracts, we utilized the most common methods in DNA binding studies: CD spectroscopy and the thermal denaturation of polynucleotides. While the former method monitors the impact of studied compounds on CD spectra of polynucleotides, the latter provides the information about the polynucleotide stabilization driven by compounds (ΔTm value). We used fluorimetry for the evaluation of the binding affinities. Competition dialysis experiment was used to probe the multitude of ligand-polynucleotide interactions. An array of different nucleic acid structures (single- stranded, double-stranded, DNA-RNA hybrid and triple helixes) were dialyzed against the compound solution for the assay. Dicationic compound 2-(2-(3-(6- carboximidamidiumbenzothiazole-2-yl)phenyl)- (benzothiazole-6-yl))-4, 5-dihydro-1H- imidazolium was identified as the most interesting one, as it showed high selectivity combined with high affinity over a particular polynucleotide structure. This compound was selected for detailed analysis with hybrid structure poly dA – poly rU by aforementioned methods.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
HRZZ-IP-2018-01-4694 - Molekularno prepoznavanje DNA:RNA hibridnih i višelančanih struktura u bioanalitičkim i in vitro sustavima (DNARNAHyB-MolBio) (Radić Stojković, Marijana, HRZZ - 2018-01) ( POIROT)
IP-2018-01-4379 - Istraživanje antioksidativnog djelovanja benzazolskog skeleta u dizajnu novih antitumorskih agensa (AntioxPot) (Hranjec, Marijana, HRZZ - 2018-01) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Tekstilno-tehnološki fakultet, Zagreb

Citiraj ovu publikaciju

Ptiček, Lucija; Racane, Livio; Zonjić, Iva; Tumir, Lidija-Marija; Radić Stojković, Marijana
Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA // Book of abstracts, 18th Ružička Days: Today science – tomorrow industry / Jukić, Ante (ur.).
Zagreb: HDKI, 2020. str. 46-46 (poster, domaća recenzija, sažetak, znanstveni)
Ptiček, L., Racane, L., Zonjić, I., Tumir, L. & Radić Stojković, M. (2020) Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA. U: Jukić, A. (ur.)Book of abstracts, 18th Ružička Days: Today science – tomorrow industry.
@article{article, editor = {Juki\'{c}, A.}, year = {2020}, pages = {46-46}, keywords = {amidines, benzazoles, interactions, DNA/RNA}, isbn = {978-953-6894-75-8}, title = {Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA}, keyword = {amidines, benzazoles, interactions, DNA/RNA}, publisher = {HDKI}, publisherplace = {Vukovar, Hrvatska} }
@article{article, editor = {Juki\'{c}, A.}, year = {2020}, pages = {46-46}, keywords = {amidines, benzazoles, interactions, DNA/RNA}, isbn = {978-953-6894-75-8}, title = {Interactions of novel amidino-substituted aryl- bis(benzazoles) with DNA/RNA}, keyword = {amidines, benzazoles, interactions, DNA/RNA}, publisher = {HDKI}, publisherplace = {Vukovar, Hrvatska} }




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