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Microchip Capillary Electrophoretic Analysis of β-Alanine and L-histidine with C4D Detector

β-Alanine and L-histidine supplementation results in elevated intramuscular carnosine content, enhancing human physical performance [1]. Thus, aforementioned amino acids are important constituent of animal feeds used in production of functional food with elevated levels of carnosine and also in various preworkout supplements. Therefore, it is necessary to develop fast and reliable analytical methods for the detection of these amino acids. Microchip capillary electrophoresis (MCE) using capacitively coupled contactless conductivity detector (C4D) in various acidic electrophoretic buffers provides high resolution and short separation time for quantitative determination of β-alanine and L-histidine. The influence of the injection and separation parameters on the separation ability was investigated. The electrophoretic buffer composition were thoroughly studied in order to optimize the separation. The linear response region for each particular amino acid investigated, both single and in a mixture, was determined using linear regression. Detection limits for β-alanine and L-histidine were below 1 mg mL-1.

amino acids ; β-alanine ; C4D detector ; L-histidine ; microchip capillary electrophoresis

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