hrvatski

Biocompatibility of fissure sealants

Introduction: The main purpose of this study was to investigate the effects of fissure sealants on buccal cells to assess their biocompatibility. Materials and methods: This study was conducted on 45 patients (27 girls and 18 boys), 7 to 16 years of age (age mean 12.09 ± 2.20). Buccal swabs (samples) were collected previous to, and also 7, 30 and 90 days after fissure sealant placement. Patients or their parents/ legal guardians filled in the questionnaire regarding demographic data (age, gender), dietary habits, health status, medication usage, and recent X-ray exposure. Results: Statistically significant difference in the number of buccal cells with condensed chromatin between T0 (time before fissure sealant placement) and T4 (90 days after fissure sealant placement) period for Helioseal F® (P = 0.025) was noticed. The other two analyzed materials did not show any significant difference. Factors such as recent X-ray exposure, medication usage, meat, candy, aerated beverages, and tea affect the development of nuclear anomalies such as condensed chromatin, whereas age induces the development of binucleated cells. Conclusion:Neither of the three tested dental sealants showed a statistically significant rise in the number of nuclear anomalies during a short period. During 90 days period, only Helioseal F® caused a statistically significant increase in the number of cells with condensed chromatin. Although these sealants are already in use, it is of high importance that their biocompatibility is constantly checked, especially in vivo clinical studies.

biocompatibility ; fissure sealants ; micronucleus assay

nije evidentirano

engleski

Biocompatibility of fissure sealants

Introduction: The main purpose of this study was to investigate the effects of fissure sealants on buccal cells to assess their biocompatibility. Materials and methods: This study was conducted on 45 patients (27 girls and 18 boys), 7 to 16 years of age (age mean 12.09 ± 2.20). Buccal swabs (samples) were collected previous to, and also 7, 30 and 90 days after fissure sealant placement. Patients or their parents/ legal guardians filled in the questionnaire regarding demographic data (age, gender), dietary habits, health status, medication usage, and recent X-ray exposure. Results: Statistically significant difference in the number of buccal cells with condensed chromatin between T0 (time before fissure sealant placement) and T4 (90 days after fissure sealant placement) period for Helioseal F® (P = 0.025) was noticed. The other two analyzed materials did not show any significant difference. Factors such as recent X-ray exposure, medication usage, meat, candy, aerated beverages, and tea affect the development of nuclear anomalies such as condensed chromatin, whereas age induces the development of binucleated cells. Conclusion:Neither of the three tested dental sealants showed a statistically significant rise in the number of nuclear anomalies during a short period. During 90 days period, only Helioseal F® caused a statistically significant increase in the number of cells with condensed chromatin. Although these sealants are already in use, it is of high importance that their biocompatibility is constantly checked, especially in vivo clinical studies.

biocompatibility ; fissure sealants ; micronucleus assay

nije evidentirano