engleski

Application of electrochemical approach and motility analysis in algal cells characterization

This study aimed to examine the influence of the morphological characteristics of green algal cells on the adhesion process and their mobility. The used model organisms were Tetraselmis suecica and (Chlorophyceae), motile microalgae of micrometer size, with different morphological characteristics of the cell envelope. The D. tertiolecta cell consists cell membrane [1, 2] enveloped by a glycoprotein cover (glycocalyx), while T. suecica cell consists of a cell membrane with complex theca made of scales, encrusted with calcium carbonate. D. tertiolecta cells have two flagella of equal length emerging from one basal body, whereas T. suecica cells have four flagella that pair out of two basal bodies. Using the electrochemical method of polarography and chronoamperometry on a dropping mercury electrode, D. tertiolecta cells were characterized based on characteristic adhesion signals occurring over a wide potential range. T. suecica cells do not adhere to the charged electrode, which is a consequence of the compact cell envelope. Using computer-assisted video microscopy and an Open Source Image Processing Software Icy (http://icy.bioimageanalysis.org/) cell swimming speed, the displacement length, and shape of their trajectories were quantified on 500 individual cells. Results showed that the speed of T. suecica cells is up to 4 times higher than that of D. tertiolecta, which is a consequence of a complex flagellar system. Based on algal cell adhesion and motility characterization, the obtained results could contribute to a better understanding of the spatial distribution of green algal cells in the aquatic environment under stress

motility ; Dunaliella ; Tetraselmis ; Cadmium ; adhesion

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