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Pregled bibliografske jedinice broj: 1078426

Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy


Margui, Eva; Dumić, Jerka; Queralt, Ignasi; Bakovic, Lada; Jablan, Jasna
Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy // Analytical methods, 12 (2020), 4899-4905 doi:10.1039/D0AY01185A (međunarodna recenzija, članak, znanstveni)


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Naslov
Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy

Autori
Margui, Eva ; Dumić, Jerka ; Queralt, Ignasi ; Bakovic, Lada ; Jablan, Jasna

Izvornik
Analytical methods (1759-9660) 12 (2020); 4899-4905

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
TXRF ; seminal plasma ; multi-elemental ; dilution ; Zn

Sažetak
Trace elements are essential for the normal spermatogenesis of mammals and play a critical role in sperm quality, but pathological processes e.g. inflammation, as well. Consequently, multi- elemental analysis of seminal plasma (SP) may provide significant information on physiological and pathophysiological processes occurring in the male reproductive tract. Therefore, the development of a simple, fast and reliable method for seminal plasma (SP) analysis by total reflection X-ray fluorescence spectrometry (TXRF) could be useful for both, scientific and clinical studies. In this study, a detailed assessment of the sample preparation parameters and measurement conditions, including analysis of the shape and element distribution of the deposited residue on the reflector by micro X- ray fluorescence spectrometry, was carried out. Using the best analytical conditions, limits of detection for trace elements were in the range of 0.04-0.3 mg/kg. Trueness and precision of the results, evaluated by spiked SP sample analysis, were in most cases acceptable with recoveries values in the range of 87-109 % and relative standard deviations 3-12% (n=5). The developed TXRF method was applied for the analysis of several SP samples from patients with different diagnoses and results were compared with those obtained by ICP-OES. Among the studied trace elements with a role in the antioxidant defence system only Zn could be quantified and some differences in Zn concentrations among studied groups were observed. However, further studies on a large number of samples are required to define the exact relationship between element composition and semen quality.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kliničke medicinske znanosti



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Opća bolnica Zadar

Profili:

Avatar Url Jerka Dumić (autor)

Avatar Url Jasna Jablan (autor)

doi

Citiraj ovu publikaciju

Margui, Eva; Dumić, Jerka; Queralt, Ignasi; Bakovic, Lada; Jablan, Jasna
Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy // Analytical methods, 12 (2020), 4899-4905 doi:10.1039/D0AY01185A (međunarodna recenzija, članak, znanstveni)
Margui, E., Dumić, J., Queralt, I., Bakovic, L. & Jablan, J. (2020) Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy. Analytical methods, 12, 4899-4905 doi:10.1039/D0AY01185A.
@article{article, year = {2020}, pages = {4899-4905}, DOI = {10.1039/D0AY01185A}, keywords = {TXRF, seminal plasma, multi-elemental, dilution, Zn}, journal = {Analytical methods}, doi = {10.1039/D0AY01185A}, volume = {12}, issn = {1759-9660}, title = {Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy}, keyword = {TXRF, seminal plasma, multi-elemental, dilution, Zn} }
@article{article, year = {2020}, pages = {4899-4905}, DOI = {10.1039/D0AY01185A}, keywords = {TXRF, seminal plasma, multi-elemental, dilution, Zn}, journal = {Analytical methods}, doi = {10.1039/D0AY01185A}, volume = {12}, issn = {1759-9660}, title = {Simple and reliable determination of Zn and some additional elements in seminal plasma samples by using total reflection X-ray fluorescence spectroscopy}, keyword = {TXRF, seminal plasma, multi-elemental, dilution, Zn} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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