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Pregled bibliografske jedinice broj: 1077595

Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach


Đaković Rode, Oktavija; Kurolt, Ivan-Christian; Puljiz, Ivan; Čivljak, Rok; Cetinić Balent, Nataša; Laškaj, Renata; Kujundžić Tiljak, Mirjana; Mikulić, Radojka; Markotić, Alemka
Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach // European journal of clinical microbiology & infectious diseases, 40 (2021), 2; 261-268 doi:10.1007/s10096-020-04019-y (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1077595 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach

Autori
Đaković Rode, Oktavija ; Kurolt, Ivan-Christian ; Puljiz, Ivan ; Čivljak, Rok ; Cetinić Balent, Nataša ; Laškaj, Renata ; Kujundžić Tiljak, Mirjana ; Mikulić, Radojka ; Markotić, Alemka

Izvornik
European journal of clinical microbiology & infectious diseases (0934-9723) 40 (2021), 2; 261-268

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
COVID-19 diagnostics ; Clinical and laboratory findings ; Anti-SARS-CoV-2 antibody response ; Serological methods ; Two-step testing approach ; Croatia

Sažetak
According to anti-SARS-CoV-2 seroresponse in patients with COVID-19 from Croatia, we emphasised the issue of different serological tests and need for combining diagnostic methods for COVID-19 diagnosis. Anti-SARS-CoV-2 IgA and IgG ELISA and IgM/IgG immunochromatographic assay (ICA) were used for testing 60 sera from 21 patients (6 with severe, 10 moderate, and 5 with mild disease). The main clinical, demographic, and haemato- biochemical data were analysed. The most common symptoms were cough (95.2%), fever (90.5%), and fatigue and shortness of breath (42.9%). Pulmonary opacities showed 76.2% of patients. Within the first 7 days of illness, seropositivity for ELISA IgA and IgG was 42.9% and 7.1%, and for ICA IgM and IgG 25% and 10.7%, respectively. From day 8 after onset, ELISA IgA and IgG seropositivity was 90.6% and 68.8%, and for ICA IgM and IgG 84.4% and 75%, respectively. In general, sensitivity for ELISA IgA and IgG was 68.3% and 40%, and for ICA IgM and IgG 56.7% and 45.0%, respectively. The anti-SARS-CoV-2 antibody distributions by each method were statistically different (ICA IgM vs. IgG, p = 0.016 ; ELISA IgG vs. IgA, p < 0.001). Antibody response in COVID-19 varies and depends on the time the serum is taken, on the severity of disease, and on the type of test used. IgM and IgA antibodies as early-stage disease markers are comparable, although they cannot replace each other. Simultaneous IgM/IgG/IgA anti-SARS-CoV-2 antibody testing followed by the confirmation of positive findings with another test in a two-tier testing is recommended.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Rijeka,
Stomatološki fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Klinika za infektivne bolesti "Dr Fran Mihaljević",
Hrvatsko katoličko sveučilište, Zagreb

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi link.springer.com

Citiraj ovu publikaciju:

Đaković Rode, Oktavija; Kurolt, Ivan-Christian; Puljiz, Ivan; Čivljak, Rok; Cetinić Balent, Nataša; Laškaj, Renata; Kujundžić Tiljak, Mirjana; Mikulić, Radojka; Markotić, Alemka
Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach // European journal of clinical microbiology & infectious diseases, 40 (2021), 2; 261-268 doi:10.1007/s10096-020-04019-y (međunarodna recenzija, članak, znanstveni)
Đaković Rode, O., Kurolt, I., Puljiz, I., Čivljak, R., Cetinić Balent, N., Laškaj, R., Kujundžić Tiljak, M., Mikulić, R. & Markotić, A. (2021) Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach. European journal of clinical microbiology & infectious diseases, 40 (2), 261-268 doi:10.1007/s10096-020-04019-y.
@article{article, author = {\DJakovi\'{c} Rode, Oktavija and Kurolt, Ivan-Christian and Puljiz, Ivan and \v{C}ivljak, Rok and Cetini\'{c} Balent, Nata\v{s}a and La\v{s}kaj, Renata and Kujund\v{z}i\'{c} Tiljak, Mirjana and Mikuli\'{c}, Radojka and Markoti\'{c}, Alemka}, year = {2021}, pages = {261-268}, DOI = {10.1007/s10096-020-04019-y}, keywords = {COVID-19 diagnostics, Clinical and laboratory findings, Anti-SARS-CoV-2 antibody response, Serological methods, Two-step testing approach, Croatia}, journal = {European journal of clinical microbiology and infectious diseases}, doi = {10.1007/s10096-020-04019-y}, volume = {40}, number = {2}, issn = {0934-9723}, title = {Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach}, keyword = {COVID-19 diagnostics, Clinical and laboratory findings, Anti-SARS-CoV-2 antibody response, Serological methods, Two-step testing approach, Croatia} }
@article{article, author = {\DJakovi\'{c} Rode, Oktavija and Kurolt, Ivan-Christian and Puljiz, Ivan and \v{C}ivljak, Rok and Cetini\'{c} Balent, Nata\v{s}a and La\v{s}kaj, Renata and Kujund\v{z}i\'{c} Tiljak, Mirjana and Mikuli\'{c}, Radojka and Markoti\'{c}, Alemka}, year = {2021}, pages = {261-268}, DOI = {10.1007/s10096-020-04019-y}, keywords = {COVID-19 diagnostics, Clinical and laboratory findings, Anti-SARS-CoV-2 antibody response, Serological methods, Two-step testing approach, Croatia}, journal = {European journal of clinical microbiology and infectious diseases}, doi = {10.1007/s10096-020-04019-y}, volume = {40}, number = {2}, issn = {0934-9723}, title = {Antibody response and the clinical presentation of patients with COVID-19 in Croatia: the importance of a two-step testing approach}, keyword = {COVID-19 diagnostics, Clinical and laboratory findings, Anti-SARS-CoV-2 antibody response, Serological methods, Two-step testing approach, Croatia} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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