The temporal dynamics of antioxidants and lipid peroxidation in chick embryo livers after low- dose gamma irradiation (CROSBI ID 282728)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vilić, Marinko ; Aladrović, Jasna ; Beer Ljubić, Blanka ; Žura Žaja, Ivona ; Gottstein, Željko ; Pejaković Hlede, Jadranka ; Majer, Marija ; Pašić, Selim
engleski
The temporal dynamics of antioxidants and lipid peroxidation in chick embryo livers after low- dose gamma irradiation
The effects were measured of low-doses of gamma radiation within 24 hours after irradiation on the activity of glutathione peroxidase (GSH- Px), superoxide dismutase (SOD), catalase (CAT) and levels of glutathione (GSH) and lipid peroxide concentrations (malondialdehyde, MDA) in embryonic chick liver. The chick embryos were irradiated with doses of gamma radiation of 0.05, 0.15, 0.3, 0.5 and 0.8 Gy at the dose rate of 0.0117 Gy/s on day 19 of incubation. All parameters were measured 1, 3, 6, 12 and 24 hours after irradiation by spectrophotometry, apart from the level of lipid peroxidation, which was assessed using high performance liquid chromatography (HPLC). A versatile analysis of the data was made and it consisted of: 1) a comparison of the means of antioxidant enzyme activities and GSH and MDA concentrations for each radiation dose and the time after irradiation with the corresponding control samples ; 2) research into the temporal dynamics of enzyme activities and concentrations a) for each value of radiation dose and b) when the data for radiation doses were merged, i.e. regardless of the radiation doses. A significant increase in GSHPx activities in time after irradiation was found in dose intervals from 0.05 to 0.5 Gy, which was well described by linear function. This is a highly reliable result, because it was obtained as a result of all the analyses applied. For other parameters, we did not find any dependence of activities and concentrations on time. The analysis of comparison of means of the irradiated and control samples gave statistically significant results for some dose-time pairs.
antioxidants ; chick ; embryo ; liver ; low-dose radiation ; gamma irradiation
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Podaci o izdanju
Povezanost rada
Veterinarska medicina