engleski

Tracking immunohistochemical neuroplastin expression in Toll-like receptor 2 deficient mice

Aim of the study was to determine the immunoreactivity pattern and unravel potential changes of two isoforms of synaptic transmembrane glycoprotein neuroplastin (Np55 and Np65) in brains of Toll-like receptor 2 deficient (TLR2D) mice in comparison with wild-type (WT) animals. For the purposes of immunohistochemical analysis by confocal microscopy, mouse brain sections derived from TLR2D and WT mice (n=10) were subjected to immunofluorescence labeling using antibodies against Np55, Np65, TLR2, and specific cell-type markers for neurons and different glial cells. Our preliminary Western blot analysis of cortical, hippocampal and cerebellar membrane fractions revealed changed expression levels of two Np isoforms in TLR2D vs WT mice. Here presented immunohistochemical data indicate higher Np55 and Np65 signal intensity in cortical and hippocampal area of TLR2D vs WT mice, and lower Np55 signal intensity in cerebellum of TLR2D mice, which corresponds to our previous Western blot results. In addition, Np was found to be expressed only in neuronal cells in both TLR2D and WT mice. TLR2D mice are a widely used model for studying neuroinflammatory component of different neuropathological disorders and Np is known to be involved in synaptic organization and affected in neurodegenerative disorders. It may be expected that deficiency of TLR2 has impact on cell-cell communication due to changed molecular signatures of neurons and surrounding glial cells. Having in mind evidenced role of TLR2D in activated microglia, deciphering biochemical phenotype of TLR2D mice could as well contribute to understanding potential involvement of neuroplastin in neuroinflammation.

neuroinflammation ; microglia

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