DNA damage in kidney and parenchymal and non- parenchymal liver cells of adult Wistar rats after subchronic oral treatment with tembotrione (CROSBI ID 282563)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Kašuba, Vilena ; Micek, Vedran ; Pizent, Alica ; Tariba Lovaković, Blanka ; Želježić, Davor ; Milić, Mirta ; Kopjar, Nevenka
engleski
DNA damage in kidney and parenchymal and non- parenchymal liver cells of adult Wistar rats after subchronic oral treatment with tembotrione
DNA damage in the liver and kidney cells of adult male Wistar rats was studied using the comet assay after a 28-day oral administration of tembotrione at doses of 0.0007, 0.0013 and 0.7 mg/kg b.w./day [AOEL (acceptable operator exposure level), REL (residual exposure level) and 1000× AOEL]. As a descriptor of DNA damage, tail intensity was used. Antioxidant status was assessed by activity of glutathione peroxidase (GPx). Significant DNA damage was recorded in the kidney cells at all three doses as compared to negative control. In parenchymal liver cells, significant DNA damage was observed in AOEL and 1000×AOEL doses, while in non- parenchymal liver cells, only AOEL-treated group was significantly different compared to negative control. In both types of liver cells, REL and 1000×AOEL doses were significantly different from the AOEL dose. No significant changes in GPx activity compared to control were observed at any exposure level. The results of the present study suggest that repeated in vivo exposure to tembotrione led to low-level DNA instability in kidney and liver cells. Exposure to the highest tembotrione dose showed a relatively weak response with the alkaline comet assay. Further research should focus on the effects of this herbicide in other models along with different exposure scenarios.
DNA damage ; Parenchymal and non-parenchymal liver cells ; Kidney cells ; Tembotrione
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
27
2020.
1800-1807
objavljeno
0944-1344
1614-7499
10.1007/s11356-019-06782-8