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Pregled bibliografske jedinice broj: 1075786

Non-invasive detection of Aphanomyces astaci on individual crayfish


Pavić, Dora; Čanković, Milan; Sviličić Petrić, Ines; Makkonen, Jenny; Hudina, Sandra; Maguire, Ivana; Vladušić, Tomislav; Šver, Lidija; Hrašćan, Reno; Orlić, Karla et al.
Non-invasive detection of Aphanomyces astaci on individual crayfish // Book of abstracts - European International Association of Astacology meeting (IAA GOTLAND 2019)
Gotland, Švedska, 2019. str. 20-20 (poster, međunarodna recenzija, sažetak, ostalo)


CROSBI ID: 1075786 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Non-invasive detection of Aphanomyces astaci on individual crayfish

Autori
Pavić, Dora ; Čanković, Milan ; Sviličić Petrić, Ines ; Makkonen, Jenny ; Hudina, Sandra ; Maguire, Ivana ; Vladušić, Tomislav ; Šver, Lidija ; Hrašćan, Reno ; Orlić, Karla ; Dragičević, Paula ; Bielen, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts - European International Association of Astacology meeting (IAA GOTLAND 2019) / - , 2019, 20-20

Skup
European International Association of Astacology meeting (IAA GOTLAND 2019)

Mjesto i datum
Gotland, Švedska, 27.-31.8.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pathogenic oomycete, native crayfish species, biofilm

Sažetak
Pathogenic oomycete Aphanomyces astaci is transmitted mainly by invasive North American crayfish, and causes the crayfish plague, a disease mostly lethal for native European crayfish. Detecting the pathogen in endangered native crayfish populations before the disease outbreak would provide a starting point in the development of effective control measures. However, current A. astaci-detection protocols either rely on eDNA isolated from large volumes of water or, if focused on individual animals, include crayfish killing. Thus, we have developed a non-invasive method that detects A. astaci DNA in microbial biofilm associated with the cuticle of individual crayfish, bypassing the unnecessary killing step. Efficiency of the new method was confirmed by PCR and qPCR and the obtained results were congruent with the traditional invasive method. We propose that the new method should be used in future monitoring of A. astaci presence in endangered European native crayfish individuals.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
HRZZ-UIP-2017-05-1720 - Promjene sastava patogena i imunološkog odgovora tijekom širenja areala uspješnih invazivnih vrsta slatkovodnih rakova (STRIVE) (Hudina, Sandra, HRZZ - 2017-05) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

www.campusgotland.uu.se

Citiraj ovu publikaciju:

Pavić, Dora; Čanković, Milan; Sviličić Petrić, Ines; Makkonen, Jenny; Hudina, Sandra; Maguire, Ivana; Vladušić, Tomislav; Šver, Lidija; Hrašćan, Reno; Orlić, Karla et al.
Non-invasive detection of Aphanomyces astaci on individual crayfish // Book of abstracts - European International Association of Astacology meeting (IAA GOTLAND 2019)
Gotland, Švedska, 2019. str. 20-20 (poster, međunarodna recenzija, sažetak, ostalo)
Pavić, D., Čanković, M., Sviličić Petrić, I., Makkonen, J., Hudina, S., Maguire, I., Vladušić, T., Šver, L., Hrašćan, R. & Orlić, K. (2019) Non-invasive detection of Aphanomyces astaci on individual crayfish. U: Book of abstracts - European International Association of Astacology meeting (IAA GOTLAND 2019).
@article{article, author = {Pavi\'{c}, Dora and \v{C}ankovi\'{c}, Milan and Svili\v{c}i\'{c} Petri\'{c}, Ines and Makkonen, Jenny and Hudina, Sandra and Maguire, Ivana and Vladu\v{s}i\'{c}, Tomislav and \v{S}ver, Lidija and Hra\v{s}\'{c}an, Reno and Orli\'{c}, Karla and Dragi\v{c}evi\'{c}, Paula and Bielen, Ana}, year = {2019}, pages = {20-20}, keywords = {pathogenic oomycete, native crayfish species, biofilm}, title = {Non-invasive detection of Aphanomyces astaci on individual crayfish}, keyword = {pathogenic oomycete, native crayfish species, biofilm}, publisherplace = {Gotland, \v{S}vedska} }
@article{article, author = {Pavi\'{c}, Dora and \v{C}ankovi\'{c}, Milan and Svili\v{c}i\'{c} Petri\'{c}, Ines and Makkonen, Jenny and Hudina, Sandra and Maguire, Ivana and Vladu\v{s}i\'{c}, Tomislav and \v{S}ver, Lidija and Hra\v{s}\'{c}an, Reno and Orli\'{c}, Karla and Dragi\v{c}evi\'{c}, Paula and Bielen, Ana}, year = {2019}, pages = {20-20}, keywords = {pathogenic oomycete, native crayfish species, biofilm}, title = {Non-invasive detection of Aphanomyces astaci on individual crayfish}, keyword = {pathogenic oomycete, native crayfish species, biofilm}, publisherplace = {Gotland, \v{S}vedska} }




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