Non-invasive detection of Aphanomyces astaci on individual crayfish (CROSBI ID 693210)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Pavić, Dora ; Čanković, Milan ; Sviličić Petrić, Ines ; Makkonen, Jenny ; Hudina, Sandra ; Maguire, Ivana ; Vladušić, Tomislav ; Šver, Lidija ; Hrašćan, Reno ; Orlić, Karla ; Dragičević, Paula ; Bielen, Ana
engleski
Non-invasive detection of Aphanomyces astaci on individual crayfish
Pathogenic oomycete Aphanomyces astaci is transmitted mainly by invasive North American crayfish, and causes the crayfish plague, a disease mostly lethal for native European crayfish. Detecting the pathogen in endangered native crayfish populations before the disease outbreak would provide a starting point in the development of effective control measures. However, current A. astaci-detection protocols either rely on eDNA isolated from large volumes of water or, if focused on individual animals, include crayfish killing. Thus, we have developed a non-invasive method that detects A. astaci DNA in microbial biofilm associated with the cuticle of individual crayfish, bypassing the unnecessary killing step. Efficiency of the new method was confirmed by PCR and qPCR and the obtained results were congruent with the traditional invasive method. We propose that the new method should be used in future monitoring of A. astaci presence in endangered European native crayfish individuals.
pathogenic oomycete, native crayfish species, biofilm
nije evidentirano
nije evidentirano
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Podaci o prilogu
20-20.
2019.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
European International Association of Astacology meeting (IAA GOTLAND 2019)
poster
27.08.2019-31.08.2019
Gotland, Švedska