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Pregled bibliografske jedinice broj: 1075548

Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells


Tominac Trcin, Mirna; Zdraveva, Emilija; Dolenec, Tamara; Vrgoč Zimić, Ivana; Bujić Mihica, Marina; Batarilo, Ivanka; Dekaris, Iva; Blažević, Valentina; Slivac, Igor; Holjevac Grgurić, Tamara et al.
Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells // Polymers, 12 (2020), 8; 1758, 26 doi:10.3390/polym12081758 (međunarodna recenzija, članak, znanstveni)


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Naslov
Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells

Autori
Tominac Trcin, Mirna ; Zdraveva, Emilija ; Dolenec, Tamara ; Vrgoč Zimić, Ivana ; Bujić Mihica, Marina ; Batarilo, Ivanka ; Dekaris, Iva ; Blažević, Valentina ; Slivac, Igor ; Holjevac Grgurić, Tamara ; Govorčin Bajsić, Emi ; Markov, Ksenija ; Čanak, Iva ; Kuzmić, Sunčica ; Tarbuk, Anita ; Tomljenović, Antoneta ; Mrkonjić, Nikolina ; Mijović, Budimir

Izvornik
Polymers (2073-4360) 12 (2020), 8; 1758, 26

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
scaffolds ; tissue engineering ; polycaprolactone ; electrospinning ; titanium dioxide ; cefuroxime ; limbal stem cell deficiency ; antimicrobial activity

Sažetak
Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Tekstilna tehnologija, Temeljne medicinske znanosti, Biotehnologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-6878 - Ciljana izrada prototipa vlaknastog nosača za uzgoj tkivnih stanica kombiniranim elektroispredanjem (COMBOELECTROSPUN) (Mijović, Budimir, HRZZ - IP-2016-06) ( POIROT)

Ustanove:
Imunološki zavod d.d.,
Medicinski fakultet, Rijeka,
Medicinski fakultet, Zagreb,
Tekstilno-tehnološki fakultet, Zagreb,
Metalurški fakultet, Sisak,
Fakultet kemijskog inženjerstva i tehnologije, Zagreb,
KBC "Sestre Milosrdnice",
Hrvatski zavod za transfuzijsku medicinu,
Centar za forenzična ispitivanja, istraživanja i vještačenja "Ivan Vučetić"

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com doi.org

Citiraj ovu publikaciju:

Tominac Trcin, Mirna; Zdraveva, Emilija; Dolenec, Tamara; Vrgoč Zimić, Ivana; Bujić Mihica, Marina; Batarilo, Ivanka; Dekaris, Iva; Blažević, Valentina; Slivac, Igor; Holjevac Grgurić, Tamara et al.
Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells // Polymers, 12 (2020), 8; 1758, 26 doi:10.3390/polym12081758 (međunarodna recenzija, članak, znanstveni)
Tominac Trcin, M., Zdraveva, E., Dolenec, T., Vrgoč Zimić, I., Bujić Mihica, M., Batarilo, I., Dekaris, I., Blažević, V., Slivac, I. & Holjevac Grgurić, T. (2020) Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells. Polymers, 12 (8), 1758, 26 doi:10.3390/polym12081758.
@article{article, author = {Tominac Trcin, Mirna and Zdraveva, Emilija and Dolenec, Tamara and Vrgo\v{c} Zimi\'{c}, Ivana and Buji\'{c} Mihica, Marina and Batarilo, Ivanka and Dekaris, Iva and Bla\v{z}evi\'{c}, Valentina and Slivac, Igor and Holjevac Grguri\'{c}, Tamara and Govor\v{c}in Bajsi\'{c}, Emi and Markov, Ksenija and \v{C}anak, Iva and Kuzmi\'{c}, Sun\v{c}ica and Tarbuk, Anita and Tomljenovi\'{c}, Antoneta and Mrkonji\'{c}, Nikolina and Mijovi\'{c}, Budimir}, year = {2020}, pages = {26}, DOI = {10.3390/polym12081758}, chapter = {1758}, keywords = {scaffolds, tissue engineering, polycaprolactone, electrospinning, titanium dioxide, cefuroxime, limbal stem cell deficiency, antimicrobial activity}, journal = {Polymers}, doi = {10.3390/polym12081758}, volume = {12}, number = {8}, issn = {2073-4360}, title = {Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells}, keyword = {scaffolds, tissue engineering, polycaprolactone, electrospinning, titanium dioxide, cefuroxime, limbal stem cell deficiency, antimicrobial activity}, chapternumber = {1758} }
@article{article, author = {Tominac Trcin, Mirna and Zdraveva, Emilija and Dolenec, Tamara and Vrgo\v{c} Zimi\'{c}, Ivana and Buji\'{c} Mihica, Marina and Batarilo, Ivanka and Dekaris, Iva and Bla\v{z}evi\'{c}, Valentina and Slivac, Igor and Holjevac Grguri\'{c}, Tamara and Govor\v{c}in Bajsi\'{c}, Emi and Markov, Ksenija and \v{C}anak, Iva and Kuzmi\'{c}, Sun\v{c}ica and Tarbuk, Anita and Tomljenovi\'{c}, Antoneta and Mrkonji\'{c}, Nikolina and Mijovi\'{c}, Budimir}, year = {2020}, pages = {26}, DOI = {10.3390/polym12081758}, chapter = {1758}, keywords = {scaffolds, tissue engineering, polycaprolactone, electrospinning, titanium dioxide, cefuroxime, limbal stem cell deficiency, antimicrobial activity}, journal = {Polymers}, doi = {10.3390/polym12081758}, volume = {12}, number = {8}, issn = {2073-4360}, title = {Poly(ε-caprolactone) titanium dioxide and cefuroxime antimicrobial scaffolds for cultivation of human limbal stem cells}, keyword = {scaffolds, tissue engineering, polycaprolactone, electrospinning, titanium dioxide, cefuroxime, limbal stem cell deficiency, antimicrobial activity}, chapternumber = {1758} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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