Impact of surface functionalization on the toxicity and antimicrobial effects of selenium nanoparticles considering different routes of entry (CROSBI ID 281689)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Galić, Emerik ; Ilić, Krunoslav ; Hartl, Sonja ; Tetyczka, Carolin ; Kasemets, Kaja ; Kurvet, Imbi ; Milić, Mirta ; Barbir, Rinea ; Pem, Barbara ; Erceg, Ina ; Dutour Sikirić, Maja ; Pavičić, Ivan ; Roblegg, Eva ; Kahru, Anne ; Vinković Vrček, Ivana
engleski
Impact of surface functionalization on the toxicity and antimicrobial effects of selenium nanoparticles considering different routes of entry
Selenium nanoparticles (SeNPs) were first designed as nutritional supplements, but they are attractive also for use in diagnostic and therapeutic systems owing to their biocompatibility and protective effects. This study aimed to examine if different SeNPs stabilization strategies affect their (i) antimicrobial activity against bacteria Escherichia coli and Staphylococcus aureus and yeast Saccharomyces cerevisiae and (ii) toxicity to human cells of different biological barriers i.e., skin, oral and intestinal mucosa. For surface stabilization, polyvinylpyrrolidone (PVP), poly-L-lysine (PLL) and polyacrylic acid (PAA) were used rendering neutral, positively and negatively charged SeNPs, respectively. The SeNPs (primary size ∼80 nm) showed toxic effects in human cells in vitro and in bacteria S. aureus, but not in E. coli and yeast S. cerevisiae. Toxicity of SeNPs (24 h IC50) ranged from 1.4 to >100 mg Se/L, depending on surface functionalization (PLL>PAA>PVP) and was not caused by ionic Se. At subtoxic concentrations, all SeNPs were taken up by all human cell types, induced oxidative stress response and demonstrated genotoxicity. As the safety profile of SeNPs was dependent not only on target cells (mammalian cells, bacteria, yeast) but also on surface functionalization, these aspects should be considered during development of novel SeNPs-based biomedical products.
antimicrobials ; medical industry ; food industry ; nanomaterials safety
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Podaci o izdanju
144
2020.
111621
11
objavljeno
0278-6915
1873-6351
10.1016/j.fct.2020.111621
Povezanost rada
Farmacija, Interdisciplinarne prirodne znanosti, Kemija