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Pregled bibliografske jedinice broj: 1072438

Surface Stabilization A ects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells


Vuković, Barbara; Marija Milić, Marija; Dobrošević, Blaženka; Milić, Mirta; Ilić, Krunoslav; Pavičić, Ivan; Šerić, Vatroslav; Vinković Vrček, Ivana
Surface Stabilization A ects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells // Nanomaterials, 10 (2020), 1390, 18 doi:10.3390/nano10071390 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1072438 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Surface Stabilization A ects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells

Autori
Vuković, Barbara ; Marija Milić, Marija ; Dobrošević, Blaženka ; Milić, Mirta ; Ilić, Krunoslav ; Pavičić, Ivan ; Šerić, Vatroslav ; Vinković Vrček, Ivana

Izvornik
Nanomaterials (2079-4991) 10 (2020); 1390, 18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
nanosafety ; surface coating ; cellular uptake ; apoptosis ; oxidative stress ; genotoxicity

Sažetak
Silver nanoparticles (AgNPs) are one of the most investigated metal-based nanomaterials. Their biocidal activity boosted their application in both diagnostic and therapeutic medical systems. It is therefore crucial to provide sound evidences for human-related safety of AgNPs. This study aimed to enhance scientific knowledge with regard to biomedical safety of AgNPs by investigating how their different surface properties affect human immune system. Methods: preparation, characterization and stability evaluation was performed for four differently coated AgNPs encompassing neutral, positive and negative agents used for their surface stabilization. Safety aspects were evaluated by testing interaction of AgNPs with fresh human peripheral blood mononuclear cells (hPBMC) by means of particle cellular uptake and their ability to trigger cell death, apoptosis and DNA damages through induction of oxidative stress and damages of mitochondrial membrane. Results: all tested AgNPs altered morphology of freshly isolated hPBMC inducing apoptosis and cell death in a dose- and time-dependent manner. Highest toxicity was observed for positively-charged and protein-coated AgNPs. Cellular uptake of AgNPs was also dose-dependently increased and highest for positively charged AgNPs. Intracellularly, AgNPs induced production of reactive oxygen species (ROS) and damaged mitochondrial membrane. Depending on the dose, all AgNPs exhibited genotoxic potential. Conclusions: this study provides systematic and comprehensive data showing how differently functionalized AgNPs may affect the human immune system. Presented results are a valuable scientific contribution to safety assessment of nanosilver-based blood-contacting medical products.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2016-06-2436 - Značaj interakcija metalnih nanočestica sa sumpornim biomolekulama za nano-bio sučelje (Ivana Vinković Vrček, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek

Citiraj ovu publikaciju

Vuković, Barbara; Marija Milić, Marija; Dobrošević, Blaženka; Milić, Mirta; Ilić, Krunoslav; Pavičić, Ivan; Šerić, Vatroslav; Vinković Vrček, Ivana
Surface Stabilization A ects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells // Nanomaterials, 10 (2020), 1390, 18 doi:10.3390/nano10071390 (međunarodna recenzija, članak, znanstveni)
Vuković, B., Marija Milić, M., Dobrošević, B., Milić, M., Ilić, K., Pavičić, I., Šerić, V. & Vinković Vrček, I. (2020) Surface Stabilization A ects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells. Nanomaterials, 10, 1390, 18 doi:10.3390/nano10071390.
@article{article, year = {2020}, pages = {18}, DOI = {10.3390/nano10071390}, chapter = {1390}, keywords = {nanosafety, surface coating, cellular uptake, apoptosis, oxidative stress, genotoxicity}, journal = {Nanomaterials}, doi = {10.3390/nano10071390}, volume = {10}, issn = {2079-4991}, title = {Surface Stabilization A ects Toxicity of Silver Nanoparticles in Human Peripheral Blood Mononuclear Cells}, keyword = {nanosafety, surface coating, cellular uptake, apoptosis, oxidative stress, genotoxicity}, chapternumber = {1390} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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