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OPTIMIZATION OF ETHANOL/WATER SOLVENT EXTRACTION OF BIOACTIVE COMPONENTS ORIGINATING FROM INDUSTRIAL HEMP (Cannabis sativa L.)

Hemp (Cannabis sativa L.) contains a wide range of biocompounds with different beneficial properties such as antiinflammatory, antithrombotic, antiarrhythmic, hypolipidemic and antioxidative. Response Surface Methodology (RSM) coupled with Box-Behnken design (BBD) was applied to determine the influence of extraction temperature, liquid to solid ratio, extraction time, rotational speed and ethanol/water solvent ratios at three levels on the solid-liquid extraction of the bioactives from the hemp (flowers, leaves, seeds, stems). Based on the obtained results, liquid to solid ratio, temperature and ethanol/water solvent ratio had statistically significant effects on the total polyphenolic content (TPC), while extraction time and rotational speed had no influence on the TPC extraction. Regarding antioxidant activity (AOX) determined by the DPPH method, only liquid to solid ratio had a statistically significant effect. Liquid to solid ratio, ethanol/water solvent ratio, temperature and rotational speed significantly influenced AOX determined by the FRAP method. According to BBD, the optimum extraction conditions were as follows: extraction temperature 45 °C, liquid to solid ratio 30 mL/g, extraction time 25 min, rotational speed 500 rpm, ethanol/water solvent ratio 25%. RSM coupled with a BBD model was shown to be effective for optimization the solid-liquid extraction of hemp.

hemp (Cannabis sativa L.) ; solid-liquid extraction ; optimization ; bioactive

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