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Pregled bibliografske jedinice broj: 1071662

PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury


Zubčić, Klara; Radovanović, Vedrana; Vlainić, Josipa; Hof, Patrick; Oršolić, Nada; Šimić, Goran; Jazvinšćak Jembrek, Maja
PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury // Oxidative medicine and cellular longevity, 2020 (2020), 9834742, 14 doi:10.1155/2020/9834742 (međunarodna recenzija, članak, znanstveni)


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Naslov
PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury

Autori
Zubčić, Klara ; Radovanović, Vedrana ; Vlainić, Josipa ; Hof, Patrick ; Oršolić, Nada ; Šimić, Goran ; Jazvinšćak Jembrek, Maja

Izvornik
Oxidative medicine and cellular longevity (1942-0900) 2020 (2020); 9834742, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
quercetin ; copper toxicity ; neurodegeneration ; pAkt ; ERK1/2 ; PUMA ; NME1 ; P19 neurons

Sažetak
Copper, a transition metal with essential cellular functions, exerts neurotoxic effects when present in excess by promoting production of reactive oxygen species (ROS). The aim of the present study was to investigate potential benefits of flavonoid quercetin against copper-induced toxicity. Results obtained with MTT assay indicate that the effects of quercetin are determined by the severity of the toxic insult. In moderately injured P19 neuronal cells, concomitant treatment with 150 μM quercetin improved viability by preventing ROS formation, caspase-3 activation, and chromatin condensation. Western blot analysis revealed that quercetin reduced copper-induced increase in p53 upregulated modulator of apoptosis (PUMA) expression and promoted upregulation of nucleoside diphosphate kinase NME1. Levels of p53 and Bax proteins were not affected by both copper and quercetin. UO126 and wortmannin, inhibitors of ERK1/2 and PI3K/Akt signalling pathways, respectively, prevented neuroprotective effects of quercetin. In severely injured neurons, 30 μM quercetin exerted strong prooxidative action and exacerbated cytotoxic effects of copper, whereas 150 μM quercetin failed to affect neuronal survival. These results demonstrate the dual nature of quercetin action in copper-related neurodegeneration. Hence, they are relevant in the context of considering quercetin as a possible therapeutic for neuroprotection and imply that detailed pharmacological and toxicological studies must be carried out for natural compounds capable of acting both as antioxidants and prooxidants.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0000000-2448 - Stres, GABA-A receptori i mehanizmi djelovanja neuropsihofarmaka (Dubravka Švob Štrac, )
GA KK01.1.1.01.0007
HKS-2018-2
HRZZ-IP-11-2013-1615
HRZZ-IP-2014-09-9730 - Hiperfosforilacija, agregacija i transsinaptički prijenos tau proteina u Alzheimerovoj bolesti: analiza likvora i ispitivanje potencijalnih neuroprotektivnih spojeva
HRZZ-IP-2019-04-3584
NIH grant AG005138

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Hrvatsko katoličko sveučilište, Zagreb

Citiraj ovu publikaciju

Zubčić, Klara; Radovanović, Vedrana; Vlainić, Josipa; Hof, Patrick; Oršolić, Nada; Šimić, Goran; Jazvinšćak Jembrek, Maja
PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury // Oxidative medicine and cellular longevity, 2020 (2020), 9834742, 14 doi:10.1155/2020/9834742 (međunarodna recenzija, članak, znanstveni)
Zubčić, K., Radovanović, V., Vlainić, J., Hof, P., Oršolić, N., Šimić, G. & Jazvinšćak Jembrek, M. (2020) PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury. Oxidative medicine and cellular longevity, 2020, 9834742, 14 doi:10.1155/2020/9834742.
@article{article, year = {2020}, pages = {14}, DOI = {10.1155/2020/9834742}, chapter = {9834742}, keywords = {quercetin, copper toxicity, neurodegeneration, pAkt, ERK1/2, PUMA, NME1, P19 neurons}, journal = {Oxidative medicine and cellular longevity}, doi = {10.1155/2020/9834742}, volume = {2020}, issn = {1942-0900}, title = {PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury}, keyword = {quercetin, copper toxicity, neurodegeneration, pAkt, ERK1/2, PUMA, NME1, P19 neurons}, chapternumber = {9834742} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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