engleski

The association between TNF-alpha, IL-1 alpha and IL-10 with Alzheimer's disease

BACKGROUND: Sporadic Alzheimer's Disease (AD) is assumed to be associated with different biological/genetic vulnerability, as well as with neuroinflammation, mediated by cytokines. The present study evaluated the role of cytokines in AD. OBJECTIVE: Aim was to determine the possible association of TNF-alpha (rs1800629), IL1- alpha (rs1800587) and IL-10 (rs1800896) polymorphisms with AD, and to assess serum TNF-alpha, IL-1alpha and IL-10 concentrations in patients with AD and in subjects with mild cognitive impairment (MCI). METHOD: The study included 645 Caucasian participants: 395 subjects with AD and 250 subjects with MCI. Genotyping was performed using real time PCR in all 645 subjects, while serum concentrations of TNF-a, IL-1alpha and 1L-10 and were determined using ELISA in 174 subjects. RESULTS: The frequency of the TNF- alpha rs1800629, IL 1-alpha rs1800587 or IL-10 rs1800896 genotypes did not differ significantly between patients with AD and MCI. Serum concentration of IL-1alpha and IL-10 were significantly decreased, while the concentration of TNF-alpha was significantly higher in patients with AD than in MCI subjects. TNF-alpha, IL1- alpha or IL-10 concentrations were similar in subjects with AD or MCI subdivided into carriers of the corresponding TNF-alpha rs1800629, IL1-alpha rs1800587 or IL-10 rs1800896 genotypes. CONCLUSIONS: Similar distribution of the IL1-alpha rs1800587, TNF- alpha rs1800629 or IL-10 rs1800896 genotypes in subjects with AD and MCI failed to confirm that these specific risk genotypes are associated with vulnerability to develop AD. Alteration in IL-1alpha, IL-10 and TNF- alpha concentrations in patients with AD partially confirmed the association with neuroinflammatory response in AD.

Alzheimer's disease ; mild cognitive impairment ; TNF-alfa rs1800629 ; IL-1-alfa rs1800587 ; IL-10 rs1800896 ; IL-1alfa ; IL-10 ; TNF-alfa

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