Metabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets (CROSBI ID 280878)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Yu, Kuai ; Matzapetakis, Manolis ; Horvatić, Anita ; Terré, Marta ; Bach, Alex ; Kuleš, Josipa ; Yeste, Natalia ; Gómez, Néstor ; Arroyo, Laura ; Rodríguez-Tomàs, Elisabet ; Peña, Raquel ; Guillemin, Nicolas ; de Almeida, André M. ; Eckersall, Peter David ; Bassols, Anna
engleski
Metabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets
In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves: 7 were fed a control diet (Ctrl) and 7 were fed the Ctrl diet supplemented with 0.3% leucine and 0.3% threonine (LT) from 5.6 days of age to 53.6 days. At this time, blood and semitendinosus muscle biopsies were collected for analysis. Integrated metabolomics and proteomics showed that branched-chain amino acids (BCAA) degradation and mitochondrial oxidative metabolism (citrate cycle and respiratory chain) were the main activated pathways in muscle because of the supplementation. BCAA derivatives and metabolites related to lipid mobilization showed the major changes. The deleterious effects of activated oxidative phosphorylation were balanced by the upregulation of antioxidant proteins. An increase in protein synthesis was indicated by elevated aminoacyl- tRNA biosynthesis and increased S6 ribosomal protein phosphorylation in skeletal muscle. In conclusion, LT group showed greater BCAA availability and mitochondrial oxidative activity ; as the muscle cells undergo greater aerobic metabolism, antioxidant defenses were activated to compensate for possible cell damage. Data are available via ProteomeXchange (PXD016098).
Amino acid ; Calf ; Muscle ; Plasma ; Metabolomics ; Proteomics
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Podaci o izdanju
216
2020.
103677
11
objavljeno
1874-3919
1876-7737
10.1016/j.jprot.2020.103677
Povezanost rada
Biotehnologija, Interdisciplinarne prirodne znanosti, Kemija, Nutricionizam, Veterinarska medicina