engleski

Diagnosis of campylobacteriosis in humans from the Manawatu, New Zealand

Campylobacteriosis is among the most frequently reported gastrointestinal diseases in the developed world. In New Zealand most diagnostic laboratories use culture methods optimised for detection of C. jejuni/coli, thus other species may be underdiagnosed. This study aimed to evaluate diagnostic methods for detection of Campylobacter species. A total of 594 faecal samples from people with clinical gastroenteritis were tested by four diagnostic methods during 2014-2015. A direct PCR targeting five Campylobacter species (Lund_PCR), 2 culture methods (CAT agar at 37°C in a hydrogen-enriched microaerobic atmosphere and mCCDA at 42°C in a microaerobic atmosphere) and the ProSpecT Campylobacter assay (ELISA) were performed on each sample. From all samples, 109 (18%) tested positive for Campylobacter spp. by at least one method. Individually, Lund_PCR detected 95 (16%), CAT 64 (11%), mCCDA 61 (10%) and ELISA 38 (6%) positive samples. Samples positive by only one method and negative by all other were 29, 11, 1 and 1 for Lund_PCR, CAT, mCCDA and EIA respectively. Only 28 samples were positive by all methods. C. jejuni was confirmed in 59 and 51 samples by mCCDA and CAT respectively. The CAT method detected 2 C. coli and 2 C. hyointestinalis samples undetected by other methods. Comparison of positive rates showed significant differences for each pair-wise combination of methods (p<0.01)except between CAT and mCCDA methods (p=0.85). The proportion of cultured campylobacters confirmed as C. jejuni was higher than that seen in studies in other countries. ELISA was the least sensitive method and not comparable to culture.

Campylobacter ; diagnosis ; prevalence ; culture ; ELISA ; PCR ; enteritis ; faeces

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