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Leukocyte Nucleolus and Anisakis pegreffii—When Falling Apart Means Falling in Place (CROSBI ID 280115)

Prilog u časopisu | ostalo | međunarodna recenzija

Mladineo, Ivona ; Hrabar, Jerko Leukocyte Nucleolus and Anisakis pegreffii—When Falling Apart Means Falling in Place // Genes, 11 (2020), 6; 688, 10. doi: 10.3390/genes11060688

Podaci o odgovornosti

Mladineo, Ivona ; Hrabar, Jerko

engleski

Leukocyte Nucleolus and Anisakis pegreffii—When Falling Apart Means Falling in Place

The view of the nucleolus as a mere ribosomal factory has been recently expanded, highlighting its essential role in immune and stress-related signalling and orchestrating. It has been shown that the nucleolus structure, formed around nucleolus organiser regions (NORs) and attributed Cajal bodies, is prone to disassembly and reassembly correlated to various physiological and pathological stimuli. To evaluate the effect of parasite stimulus on the structure of the leukocyte nucleolus, we exposed rat peripheral blood mononuclear cells (PBMC) to the crude extract of the nematode A. pegreffii (Anisakidae), and compared the observed changes to the effect of control (RPMI-1640 media), immunosuppressive (MPA) and immunostimulant treatment (bacterial lipopolysaccharide (LPS) and viral analogue polyinosinic:polycytidylic acid (poly I:C)) by confocal microscopy. Poly I:C triggered the most accentuated changes such as nucleolar fragmentation and structural unravelling, LPS induced nucleolus thickening reminiscent of cell activation, while MPA induced disassembly of dense fibrillar and granular components. A. pegreffii crude extract triggered nucleolar segregation, expectedly more enhanced in treatment with a higher dose. This is the first evidence that leukocyte nucleoli already undergo structural changes 12 h post-parasitic stimuli, although these are likely to subside after successful cell activation.

Anisakis pegreffii ; nucleolus ; nucleolar stress ; peripheral blood mononuclear leukocytes ; Sprague Dawley rat

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Podaci o izdanju

11 (6)

2020.

688

10

objavljeno

2073-4425

10.3390/genes11060688

Povezanost rada

Biologija, Biotehnologija, Veterinarska medicina

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