engleski

Study of bactericidal effect of laser synthetized silver nanoparticles: A novel model for E. coli growth and inactivation

Study of bactericidal effect of silver nanoparticles (AgNPs), free of chemical byproducts, could be of great importance in understanding the underlying antibacterial mechanism(s). In this work, we present the production and characterization of AgNPs, synthesized by laser ablation of silver in water, and optical density (OD) growth curves of E. coli cells treated at sub MIC (minimal inhibitory concentration) and MIC concentrations. Besides the increase of the baseline and reduction of the maximal OD, the main impact of the treatment is the increase of the lag time, which becomes infinitive for the MIC treatment. The obtained bacterial growth curves are described by a novel growth and inactivation model given by the set of three differential equations. Our model fits the OD data very well and explains the apparent lag phase, which we found to be a dynamic state in which the bacterial growth and death rates are close in value.

laser synthesis of nanoparticles ; silver nanoparticles ; antibacterial activity ; modeling bacterial growth

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