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The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage


Radić, Martina; Šoštar, Marko; Weber, Igor; Ćetković, Helena; Slade, Neda; Herak Bosnar, Maja
The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage // International journal of molecular sciences, 21 (2020), 7; 2363-2382 doi:10.3390/ijms21072363 (međunarodna recenzija, članak, znanstveni)


Naslov
The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage

Autori
Radić, Martina ; Šoštar, Marko ; Weber, Igor ; Ćetković, Helena ; Slade, Neda ; Herak Bosnar, Maja

Izvornik
International journal of molecular sciences (1422-0067) 21 (2020), 7; 2363-2382

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
NME ; NDPK ; Nm23 ; nucleoside diphosphate kinase ; FRET ; FLIM ; live-cell imaging

Sažetak
Nucleoside diphosphate kinases (NDPK/NME/Nm23) are enzymes composed of subunits NME1/NDPK A and NME2/NDPK B, responsible for the maintenance of the cellular (d)NTP pool and involved in other cellular processes, such as metastasis suppression and DNA damage repair. Although eukaryotic NDPKs are active only as hexamers, it is unclear whether other NME functions require the hexameric form, and how the isoenzyme composition varies in different cellular compartments. To examine the effect of DNA damage on intracellular localization of NME1 and NME2 and the composition of NME oligomers in the nucleus and the cytoplasm, we used live-cell imaging and the FRET/FLIM technique. We showed that exogenous NME1 and NME2 proteins co-localize in the cytoplasm of non-irradiated cells, and move simultaneously to the nucleus after gamma irradiation. The FRET/FLIM experiments imply that, after DNA damage, there is a slight shift in the homomer/heteromer balance between the nucleus and the cytoplasm. Collectively, our results indicate that, after irradiation, NME1 and NME2 engage in mutual functions in the nucleus, possibly performing specific functions in their homomeric states. Finally, we demonstrated that fluorophores fused to the N- termini of NME polypeptides produce the largest FRET effect and thus recommend this orientation for use in similar studies.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-1615 - Otkrivanje novih proteinskih interakcija kao podloga za nove pristupe liječenju melanoma čovjeka (Neda Slade, )
HRZZ-IP-2016-06-4021 - Struktura, funkcija i evolucija proteina Nme6/Nm23-H6 (Maja Herak Bosnar, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Radić, Martina; Šoštar, Marko; Weber, Igor; Ćetković, Helena; Slade, Neda; Herak Bosnar, Maja
The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage // International journal of molecular sciences, 21 (2020), 7; 2363-2382 doi:10.3390/ijms21072363 (međunarodna recenzija, članak, znanstveni)
Radić, M., Šoštar, M., Weber, I., Ćetković, H., Slade, N. & Herak Bosnar, M. (2020) The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage. International journal of molecular sciences, 21 (7), 2363-2382 doi:10.3390/ijms21072363.
@article{article, year = {2020}, pages = {2363-2382}, DOI = {10.3390/ijms21072363}, keywords = {NME, NDPK, Nm23, nucleoside diphosphate kinase, FRET, FLIM, live-cell imaging}, journal = {International journal of molecular sciences}, doi = {10.3390/ijms21072363}, volume = {21}, number = {7}, issn = {1422-0067}, title = {The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage}, keyword = {NME, NDPK, Nm23, nucleoside diphosphate kinase, FRET, FLIM, live-cell imaging} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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