hrvatski

De novo expression of transfected Sirt3 enhances susceptibility of human MCF-7 breast cancer cells to hyperoxia treatment

Sirtuin 3 (Sirt3) has a promising role in cancer treatment, but there are controversies about its role in different types of cancer. Since its expression is reduced in many breast cancer cells and is associated with the production of reactive oxygen species (ROS), and hyperoxia supresses growth of certain mammary carcinoma cells, we studied the effect of de novo Sirt3 expression in human MCF-7 breast cancer cells upon 44 h long hyperoxic treatment. Sirt3 effects were enhanced upon hyperoxia: decreased metabolic activity, cellular growth and senescence, reduced expression of pro-angiogenic genes, induced metabolic switch from glycolysis towards OXPHOS, induced DNA damage, mitochondrial and antioxidant dysfunction, upregulated p53 and ROS levels. The mitigation of tumorigenic properties of MCF-7 cells and enhancement of their susceptibility to the hyperoxic treatment upon de novo Sirt3 expression indicates the promising therapeutic effect of these factors in breast cancer malignancies.

hyperoxia ; sirtuin 3 ; breast cancer ; mitochondria ; oxidative stress

nije evidentirano