engleski

Anti-adhesive action of novel ruthenium(II) chlorophenyl terpyridine complexes with a high affinity for double-stranded DNA: in vitro and in silico

Interactions of three Ru(II) chlorophenyl terpyridine complexes, i.e. [Ru(Cl-Ph-tpy) (en)Cl]Cl (1), [Ru(Cl-Ph-tpy)(dach)Cl]Cl (2) and [Ru(Cl-Ph-tpy)(bpy)Cl]Cl (3) (Cl-Ph-tpy = 4′-(4-chlorophenyl)-2, 2′:6′, 2′′-terpyridine, en = 1, 2-diaminoethane, dach = 1, 2- diaminocyclohexane, bpy = 2, 2′-bipyridine) with human serum albumin, calf thymus DNA and a double helical oligonucleotide 1BNA were examined. Fluorescence emission studies were used to assess the interactions of complexes with human serum albumin, which were of moderate strength for 1 and 2. Molecular docking allowed us to predict mostly π-π stacking and van der Waals interactions between the complexes and the protein. We suggest that the complexes bind to a novel site on human albumin, which is different from its druggable sites I, II or III. Finally, when applied to normal extravillous cell line STR8/SVneo and JAr choriocarcinoma cell line, complexes 1 and 2 exerted anti-adhesive properties at very low doses, whereas complex 3 had a negligible effect. The presented results are a completion of our studies of Ru(II) complexes with terpyridine ligands and suggest a new research direction regarding cellular effects of Ru(II) polypyridyl compounds.

Ru(II) chlorophenyl terpyridine compounds ; HSA binding site ; partial intercalation ; DNA binding ; linear dichroism ; anti-adhesive properties

nije evidentirano