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Pregled bibliografske jedinice broj: 1059521

Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma


Wagner, Jasenka; Džijan, Snježana; Pavan-Jukić, Doroteja; Wagner, Jadranka; Lauc, Gordan
Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma // Prenatal Diagnosis, 28 (2008), 5; 412-416 doi:10.1002/pd.1986 (međunarodna recenzija, članak, znanstveni)


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Naslov
Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma

Autori
Wagner, Jasenka ; Džijan, Snježana ; Pavan-Jukić, Doroteja ; Wagner, Jadranka ; Lauc, Gordan

Izvornik
Prenatal Diagnosis (0197-3851) 28 (2008), 5; 412-416

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell-free fetal DNAPolymorphic STR lociPrenatal gender determinationY-chromosome DNA

Sažetak
Objective Aiming to develop more reliable methods for determination of fetal gender from maternal plasma we compared three different systems of polymerase chain reaction (PCR) detection of Y- chromosome DNA. Methods Cell- free DNA was isolated from 96 samples of maternal plasma and (1) amplified using AmpFLSTR-Identifiler (15 autosomal STR loci and amelogenin) or AmpFLSTR- Yfiler (16 Y-chromosome STR loci) kits and subsequently analyzed on ABI-PRISM 310 Genetic Analyzer, or (2) analyzed using Quantifiler-Y DNA-Quantification kit. Gender of fetuses was confirmed by cytogenetic analysis or pheno- typically at birth. Results and Conclusions AmpFLSTR-Identifiler and Quantifiler-Y Human- Quantification kits were rather reliable in determining fetal gender (92.5 and 98.1%, respectively), but false negatives were still present in both systems. AmpFLSTR-Yfiler was found to be fully reliable as it amplified Y- chromosome in all cases of male fetuses, and was thus 100% correct in determining fetal gender. In addition, it enabled comparison of polymorphic Y- chromosome loci between father and a child, thus further supporting specificity of obtained results. Copyright © 2008 John Wiley & Sons, Ltd.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove:
Klinička bolnica "Merkur"

doi

Citiraj ovu publikaciju

Wagner, Jasenka; Džijan, Snježana; Pavan-Jukić, Doroteja; Wagner, Jadranka; Lauc, Gordan
Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma // Prenatal Diagnosis, 28 (2008), 5; 412-416 doi:10.1002/pd.1986 (međunarodna recenzija, članak, znanstveni)
Wagner, J., Džijan, S., Pavan-Jukić, D., Wagner, J. & Lauc, G. (2008) Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma. Prenatal Diagnosis, 28 (5), 412-416 doi:10.1002/pd.1986.
@article{article, year = {2008}, pages = {412-416}, DOI = {10.1002/pd.1986}, keywords = {Cell-free fetal DNAPolymorphic STR lociPrenatal gender determinationY-chromosome DNA}, journal = {Prenatal Diagnosis}, doi = {10.1002/pd.1986}, volume = {28}, number = {5}, issn = {0197-3851}, title = {Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma}, keyword = {Cell-free fetal DNAPolymorphic STR lociPrenatal gender determinationY-chromosome DNA} }
@article{article, year = {2008}, pages = {412-416}, DOI = {10.1002/pd.1986}, keywords = {Cell-free fetal DNAPolymorphic STR lociPrenatal gender determinationY-chromosome DNA}, journal = {Prenatal Diagnosis}, doi = {10.1002/pd.1986}, volume = {28}, number = {5}, issn = {0197-3851}, title = {Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma}, keyword = {Cell-free fetal DNAPolymorphic STR lociPrenatal gender determinationY-chromosome DNA} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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