engleski

Biomarkers detected by the infrared spectroscopy in spent media metabolomes of embryo-derived teratomas treated with valproate in vivo

Spectroscopy screening of metabolomes detect biological activity of the epigenetic-valproate - derived teratomas in vivo.Principal component analysis of FTIR results showed that spent media metabolomes formed well‐separated clusters reflecting the treatment and day of cultivation. In metabolomes of VPA‐treated teratomas, we found elevation of previously described histone acetylation biomarkers [amide I α‐helix and A(CH3)/A(CH2)]) with apoptotic biomarkers within the amide I region for β‐sheets, and unordered and CH2 vibrations of lipids. VPA may be proposed for therapy of the undifferentiated component of teratoma tumors and this biological system completed by metabolome analysis, for a faster dual screening of antitumor/embryotoxic agents.

embryo ; teratomas ; spectrosccopy

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