engleski

Histidine dipeptides and its derivatives: analytical methods for biological and food samples

Histidine dipeptides and its derivatives have a significant role in a variety of physiological and pathological processes. For example, carnosine possesses great antioxidant, protective, chelating and anti-glycation activities. Consequently, these compounds play one of the central roles in modern supplement and nutrition research. Thus it is important to determine the intake of these compounds and benefits obtained by their consumption. The presence of specific histidine dipeptides as well as their specific concentration ratios can also be extended to the food fraud prevention process. Given the increasing interest in the research of histidine dipeptides extensive attention has been dedicated to the development of analytical techniques for their characterization and quantification in various medical and food samples. This chapter discusses available analytical methods for determination of various bioactive histidine dipeptides and its derivatives, their advantages and disadvantages as well as their specific applications. Modern analytical methods like liquid chromatography (LC) and capillary electrophoresis (CE) are revised and discussed. Special attention is placed on the microfluidic methods. The use of NMR spectroscopy has been also examined as a method to evaluate meat markers intake. Electroanalytical methods provide an excellent knowledge on histidine dipeptides and its derivatives such as kinetics of electron transfer, diffusion coefficients, oxidation mechanism, the influence of pH, adsorption processes and limit of detection of this electroactive biomolecules. Sample pre- treatment methods are also discussed.

anserine, carnosine, CE (capillary electrophoresis), electroanalytical methods, histidine dipeptides, LC (liquid chromatography)

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