engleski

Pentapeptide BPC 157 and its role in corneal epithelial wounds healing in rats

Purpose. To evaluate the role of BPC 157 in corneal epithelial wounds healing in rats. Methods. 92 albino Wistar rats, 200 g b.w. were divided in 4 groups ( A, B, C, D ) each containing 23 animals. After intraperitoneal and topical anesthesia, circular lesions were induced to the right eye by abrading corneal epithelium with surgical lancet under surgical microscope leaving 1 mm of non-abraded epithelial ring adjacent to the limbus. Wounds were stained with standard florescein and photographed. Immediately after wounds were induced and photographed, the animals were administered eye drops as follows: group A (control group) - 0.9 % NaCl (2 drops), group B - BPC 157 2pg/ml dissolved in 0.9% NaCl (2 drops), group C- BPC 157 2 ng/ml in 0.9% NaCl (2 drops), group D-BPC 157 2&micro ; g in 0.9% NaCl (2 drops). Drops were applied during a 24 h period at 0 h, 8 h, 16 h, 24 h. Lesions were stained and photographed every 8 hours: at 0 hours (induction), 8 h, 16 h, 24 h and 32 h after induction prior to treatment. The pictures were analyzed using VAMS , an image analysis system. The size of the epithelial wound area at a particular time (8, 16, 24, 32 h) was shown as a percentage of the wound area size at time 0h (100 % -the basic starting value) for every animal in every group. Data was then compared between groups to see whether there was a difference in healing area size. Results. Quite extensive lesions were induced in all rats before therapy initiation. Even at the earliest assessment point 8 hours after injury was induced, lesions became smaller, in all groups. When compared to the control group lesions were significantly smaller in groups C and D. Also assessed at later intervals at 16 h, 24 h and 32 h after injury was induced, lesions were significantly smaller in pentadecapeptide BPC 157 rats treated with &micro ; g and ng solutions (groups C and D). Conclusion. BPC 157 was shown to be effective in promoting corneal wounds healing in rats. The results were dose dependent.

pentapeptide BPC 157; wound healing; cornea; rats

Rad je kao predavanje prezentiran i na skupu International Symposium on ocular immunology, inflamation & transplantation, održanom od 22.-24.09.2002., London, Velika Britanija, objavljen u Knjizi sažetaka, str. 32-32 ; a kao poster na skupu ARVO Annual Meeting, održanom od 04.-09.05.2003., Fort Lauderdale, Florida, SAD, također objavljen u Knjizi sazetaka, str. 158-158.