engleski

Associations of TNF alpha-308G>A Genetic Polymorphism with Allergic Diseases

This study was performed on 356 Croatian students. The diagnosis of atopic asthma (AA), rhinitis (AR) and dermatitis (AD) was based on self-reported symptoms by the modified International Study of Asthma and Allergies in Childhood questionnaire and a positive standard skin prick test (SPT) to at least one common inhalation allergen. The diagnosis of contact allergy was based on a self-reported skin symptoms and positive standard patch test (European baseline series) to at least one tested contact allergen (subgroup of 312 students was patch tested). Genetic polymorphisms were genotyped using the polymerase chain reaction- based technique. Multivariate logistic regression models were adjusted for personal and lifestyle factors (for atopic disease models: sex, history of atopy in parents, Continental vs. Mediterranean residency, and pet ownership in childhood ; for contact sensitization models: sex and atopy). Results: Compared to the control subjects, there was a significant negative association of the TNFα –308G>A polymorphism with AA, AD, and separately for skin symptoms and positive SPT. These observations were confirmed in a multivariate model only for AD and skin symptoms (atopic dermatitis: OR = 0.27 ; 95% CI 0.07–1.00 ; p = 0.050 ; skin symptoms: OR = 0.29 ; 95% CI 0.10–0.83 ; p = 0.021). Based on multivariate analysis, TNFα 308G>A polymorphism was confirmed as a predictor of contact sensitization to p- phenylenediamine (OR: 5.72 ; 95% CI 1.20-27.28).

TNF ; genetic polymorpism ; allergic diseases

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