engleski

The comparison of east Croatia autosomal STR frequencies with general Croatian and neighbouring populations

Introduction: We aimed to compare the genetic profile of Eastern Croatian population by studying differences in short tandem repeat (STR) allele frequency between unrelated volunteers from 5 eastern Croatian counties (n=101), general Croatian population (n=195) and four neighbouring countries (Serbia (n=365), Bosnia and Herzegovina (n=1000), Hungary (n=4213) and Montenegro (n=101)). Materials and methods: Peripheral blood was sampled on FTA cards, and genomic DNA extracted with the use of Chelex method. AmpFlSTR® Identifiler Plus kit and ProFlex 3x32 well PCR system were used for the multiplex amplification of 15 STR loci including D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA. Allelic variants were identified by electrophoresis on an ABI 310 Genetic Analyser and GeneMapper ID v3.2.1 was used for allele annotation. Allelic frequencies were obtained by direct counting and the exact test of population differentiation was calculated using Markov-chain algorithm and Arlequin v3.5.2.2 software. Results: In total, 129 STR alleles were detected in the Eastern Croatian population, 8 of which qualifying as rare variants (frequency<0.005). The significant differences between eastern Croats, general Croatian (p<0.0001), Serbian (p<0.0001) and Montenegrin (p<0.0001) population were noticed at D2S1338 STR locus. The CSF1PO allele frequency was in addition, significantly different in Hungarians (p=0.03684) and Serbs (p=0.0306), while Montenegrins also exhibited different D18S51 (p<0.0001) allelic frequency. The STR frequency comparison between east Croatia and Bosnia and Herzegovina, revealed no differences. Conclusion: Compared to East Croatia, neighbouring populations exhibit different patterns of allelic frequency at 6 examined STR loci, 4 of which determined in comparison to Serbia and Montenegro. Phylogenetic and correspondence analysis should be performed in addition for more detailed characterisation of population relationships.

DNA profiling ; STR ; East Croatia

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