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Pregled bibliografske jedinice broj: 1053012

Heparanase binds substrate via a dynamic binding mechanism


Ahmed, Hafna; Maršavelski, Aleksandra; Moahmed, Elaaf; Jamieson, Emily; Correy, Galen; Jackson, Colin
Heparanase binds substrate via a dynamic binding mechanism // Molecular Biophysics – ABC of the puzzle of Life, ARBRE-MOBIEU Plenary Meeting / Ivošević DeNardis, Nadica ; Campos-Olivas, Ramon ; Miele, E. Miele ; England, Patrick ; Vuletić, Tomislav (ur.).
Zagreb: Ruđer Bošković Institute and Croatian Biophysical Society, 2019. str. 104-105 (poster, podatak o recenziji nije dostupan, sažetak, ostalo)


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Naslov
Heparanase binds substrate via a dynamic binding mechanism

Autori
Ahmed, Hafna ; Maršavelski, Aleksandra ; Moahmed, Elaaf ; Jamieson, Emily ; Correy, Galen ; Jackson, Colin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

ISBN
978-953-7941-28-4

Skup
Molecular Biophysics – ABC of the puzzle of Life, ARBRE-MOBIEU Plenary Meeting

Mjesto i datum
Zagreb, Hrvatska, 18-20.03.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Human heparanase, heparan sulfate, MD simulations

Sažetak
Human heparanase (HPSE) cleaves heparan sulfate (HS) localized in the extracellular matrix allowing immune response to occur as well as cell proliferation and angiogenesis. On the other hand, increased HPSE activity promotes a variety of pathological conditions such as arterial thrombosis and tumor metastasis[1], making HPSE an important drug target. Although crystal structures of HSPE are available[2], the nature of interactions with its very flexible and highly negatively charged polysaccharide substrate HS is not fully understood. To better understand processes that govern substrate recognition, we have explored the ligand binding mechanism of HPSE by analysing its conformational landscape with molecular dynamics (MD) simulations and multi-conformer models generated by crystallographic ensemble refinement. Our results imply that HS binding to HPSE is a complex process. Heparan sulfate is stabilized and immobilized at the active site, whereas the outer regions of the enzyme binding site in complex with HS are characterized with dynamic ionic interactions and exhibit substantial conformational flexibility (Fig. 1). This dynamic substrate binding mechanism allows HSPE to retain high affinity towards its substrate while minimizing the entropic costs associated with binding highly flexible molecules.[3]

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Aleksandra Maršavelski (autor)

Citiraj ovu publikaciju

Ahmed, Hafna; Maršavelski, Aleksandra; Moahmed, Elaaf; Jamieson, Emily; Correy, Galen; Jackson, Colin
Heparanase binds substrate via a dynamic binding mechanism // Molecular Biophysics – ABC of the puzzle of Life, ARBRE-MOBIEU Plenary Meeting / Ivošević DeNardis, Nadica ; Campos-Olivas, Ramon ; Miele, E. Miele ; England, Patrick ; Vuletić, Tomislav (ur.).
Zagreb: Ruđer Bošković Institute and Croatian Biophysical Society, 2019. str. 104-105 (poster, podatak o recenziji nije dostupan, sažetak, ostalo)
Ahmed, H., Maršavelski, A., Moahmed, E., Jamieson, E., Correy, G. & Jackson, C. (2019) Heparanase binds substrate via a dynamic binding mechanism. U: Ivošević DeNardis, N., Campos-Olivas, R., Miele, E., England, P. & Vuletić, T. (ur.)Molecular Biophysics – ABC of the puzzle of Life, ARBRE-MOBIEU Plenary Meeting.
@article{article, year = {2019}, pages = {104-105}, keywords = {Human heparanase, heparan sulfate, MD simulations}, isbn = {978-953-7941-28-4}, title = {Heparanase binds substrate via a dynamic binding mechanism}, keyword = {Human heparanase, heparan sulfate, MD simulations}, publisher = {Ru\djer Bo\v{s}kovi\'{c} Institute and Croatian Biophysical Society}, publisherplace = {Zagreb, Hrvatska} }
@article{article, year = {2019}, pages = {104-105}, keywords = {Human heparanase, heparan sulfate, MD simulations}, isbn = {978-953-7941-28-4}, title = {Heparanase binds substrate via a dynamic binding mechanism}, keyword = {Human heparanase, heparan sulfate, MD simulations}, publisher = {Ru\djer Bo\v{s}kovi\'{c} Institute and Croatian Biophysical Society}, publisherplace = {Zagreb, Hrvatska} }




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