engleski

Instrumental techniques in vitamin C analysis

Vitamin C is water-soluble and the first synthesized vitamin, which has significant antiradical properties and the positive influence on human health. Usually, the content of vitamin C in real samples is determined by the potentiometric and iodometric titration, spectrophotometry and electrochemical techniques. Because of the rapid oxidation on the surface of the glassy carbon electrode, voltammetric techniques are also a good choice for determination of vitamin C content in the real samples as well chronopotentiometry. The aim of this paper is to show the application of the most common titration techniques and spectrophotometry in comparison to electrochemical techniques such as cyclic, differential pulse and square-wave voltammetry and flow chronopotentiometry in the determination of vitamin C content in real samples (fruits, juices and tablets of multivitamins). The linearity of methods, limits of detection and limits of quantification for all applied instrumental techniques were determined and all techniques proved to be sensitive and selective for determination of the vitamin C content in the real samples.

vitamin C ; spectrophotometry ; titrations ; electrochemical techniques ; real samples

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