engleski

Assembly of a Complete Mitogenome of Balkan Chamois (Rupicapra rupicapra balcanica)

Mitochondrial DNA (mitogenome) is a good genetic marker for genetic variability analysis and for inferring evolutionary patterns. Mitogenome comparisons are used as tools in modern taxonomic studies since the taxonomic relationships between animal taxa have been traditionally defined based on geography and morphology. Northern chamois (Rupicapra rupicapra) is a mountain ungulate that is currently categorized into seven subspecies, but some genetic studies contradict this taxonomy. Two subspecies of Northern chamois inhabit Croatia: Balkan (Rupicapra rupicapra balcanica) and Alpine chamois (Rupicapra rupicapra rupicapra). In this study, we report the assembly of a complete mitogenome sequence of the Balkan chamois. The new sequence was directly assembled from Illumina next- generation sequencing short reads that were aligned to a reference genome of Northern chamois since they share approximately 99.8% mitogenome similarity. The newly assembled mitogenome is 16 443 bp in length and contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and D-loop region. The mitogenome is AT- biased with a nucleotide composition of 33.5% A, 30.0% T, 23.2% C, and 13.4% G. The assembly of the complete mitogenome sequence provided here, along with mitogenomes of other Northern chamois subspecies, is the initial step in resolving the taxonomy of the Genus Rupicapra.

mitogenome, reference aided assembly, chamois, taxonomy

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