engleski

Software assisted solvent screening and extraction of bioactive compounds from Mediterranean plants with “green“ solvents

Choice of an appropriate solvent is among the crucial steps in the development of a liquid‐liquid extraction method. The procedure can be simplified by using software for modeling and evaluation of thermodynamic properties such as COSMO-RS, which calculates a multitude of solvation parameters in the absence of experimental data. The aim of this study was to extract bioactive components from sage (Salvia officinalis L.) and oregano (Origanum vulgare L.) by solvent extraction and high power ultrasound extraction and thus experimentally evaluate the predicted solubilities of chosen bioactive components as determined by COSMO-RS and Hansen software. Samples of sage and oregano were treated by means of high power ultrasound with differing amplitude rates. Extraction of treated and untreated samples was performed in various timeframes with the following “green” solvents: water, 25 % and 50 % ethanol, ethyl acetate, dimethyl carbonate, limonene and α-pinene. Largest yield of total phenolics was obtained with oregano samples that were treated for 3 min, using 100% amplitude in 25 % ethanol solution. Antioxidant activity is overall increased with increased ultrasound amplitude, while being highly dependent on temperature, time and extraction solvent choice. Highest antioxidant activity is noticed with 50% ethanol as solvent. The ultrasound-aided extraction method has shown higher antioxidant activity of the samples, as compared to the samples that were subjected to the conventional extraction method.

sage ; oregano ; COSMO-RS ; solubility prediction ; antioxidant activity

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