engleski

Characterization of plant growth promoting rhizobacteria and their benefits on soybean growth

The study presents the results of genotypic and phenotypic characterization of plant growth promoting (PGP) bacteria isolated from various soybean cultivars. A total of 18 isolates were isolated from two different soybean cultivars (AFZG Ana and Gabriela). Morphological characterisation has shown that all of the isolates were rod-shaped gram negative bacteria. The sequencing has shown that 12 out of 18 isolates belong to the genus Pseudomonas, four of which belong to P. fluorescens species. Isolates which belong to the Pseudomonas genus have shown the highest ability of indole-3-acetic acid synthesis, phosphorous solubilization, and along with isolates SGN6, SGN7 and SGS4 (Sphingomonas sanguinis) potassium solubilisation too. All the isolates from Pseudomonas genus as well as those belonging to B. japonicum species have shown the protease synthesis abilities while amylase synthesis abilities was observed only in the isolate SAK2 (P. chlororaphis). The most efficient strains in in vitro biological nitrogen fixation assay belong to Pseudomonas and Rhizobium genera. Most of the isolates were positive to the acetic acid production while all of them have shown the ability of the exopolysaccharide production.

PGPR, soybean, in vitro assay, characterization, rrs gene sequencing

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