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Pregled bibliografske jedinice broj: 1049845

AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients


Brčić, Luka; Barić, Ana; Benzon, Benjamin; Brekalo, Marko; Gračan, Sanda; Kaličanin, Dean; Škrabić, Veselin; Zemunik, Tatijana; Barbalić, Maja; Novak, Ivana et al.
AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients // Scientific reports, 10 (2020), 1754, 11 doi:10.1038/s41598-020-58457-x. (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1049845 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients

Autori
Brčić, Luka ; Barić, Ana ; Benzon, Benjamin ; Brekalo, Marko ; Gračan, Sanda ; Kaličanin, Dean ; Škrabić, Veselin ; Zemunik, Tatijana ; Barbalić, Maja ; Novak, Ivana ; Pešutić Pisac, Valdi ; Punda, Ante ; Boraska Perica, Vesna

Izvornik
Scientific reports (2045-2322) 10 (2020); 1754, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Hashimoto’s thyroiditis ; AATF ; SMARCA2 ; genome-wide association study (GWAS)

Sažetak
Thyroid volume of Hashimoto’s thyroiditis (HT) patients varies in size over the course of disease and it may refect changes in biological function of thyroid gland. Patients with subclinical hypothyroidism predominantly have increased thyroid volume whereas patients with more pronounced hypothyroidism have smaller thyroid volumes. Suggested mechanism for thyroid atrophy is thyrocyte death due to apoptosis. We performed the frst genome-wide association study (GWAS) of thyroid volume in two groups of HT patients, depending on levothyroxine (LT4) therapy, and then meta- analysed across. Study included 345 HT patients in total and 6 007 322 common autosomal genetic variants. Underlying hypothesis was that genetic components that are involved in regulation of thyroid volume display their efect in specifc pathophysiologic conditions of thyroid gland of HT patients. We additionally performed immunohistochemical analysis using thyroid tissues and analysed diferences in expression levels of identifed proteins and apoptotic marker between HT patients and controls. We found genome-wide signifcant association of two loci, both involved in apoptosis, with thyroid volume of HT patients: rs7212416 inside apoptosis- antagonizing transcription factor AATF (P=8.95×10−9 ) and rs10738556 near chromatin-remodeling SMARCA2 (P=2.83×10−8 ). In immunohistochemical analysis we observed that HT patients with homozygous AATF risk genotypes have decreased AATF expression (0.46-fold, P<0.0001) and increased apoptosis (3.99-fold, P=0.0001) in comparison to controls. HT patients with heterozygous SMARCA2 genotypes have decreased SMARCA2 expression, albeit without reaching statistical signifcance (1.07-fold, P=0.5876), and signifcantly increased apoptosis (4.11-fold, P <0.0001). By two lines of evidence we show that two highly plausible genetic loci, AATF and SMARCA2, may be involved in determining the thyroid volume of HT patients. The results of our study signifcantly add to the current knowledge of disturbed biological mechanisms in thyroid gland of HT patients.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ 4950

Ustanove:
KBC Split,
Medicinski fakultet, Split

Poveznice na cjeloviti tekst rada:

doi www.ncbi.nlm.nih.gov www.nature.com doi.org

Citiraj ovu publikaciju:

Brčić, Luka; Barić, Ana; Benzon, Benjamin; Brekalo, Marko; Gračan, Sanda; Kaličanin, Dean; Škrabić, Veselin; Zemunik, Tatijana; Barbalić, Maja; Novak, Ivana et al.
AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients // Scientific reports, 10 (2020), 1754, 11 doi:10.1038/s41598-020-58457-x. (međunarodna recenzija, članak, znanstveni)
Brčić, L., Barić, A., Benzon, B., Brekalo, M., Gračan, S., Kaličanin, D., Škrabić, V., Zemunik, T., Barbalić, M. & Novak, I. (2020) AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients. Scientific reports, 10, 1754, 11 doi:10.1038/s41598-020-58457-x..
@article{article, author = {Br\v{c}i\'{c}, Luka and Bari\'{c}, Ana and Benzon, Benjamin and Brekalo, Marko and Gra\v{c}an, Sanda and Kali\v{c}anin, Dean and \v{S}krabi\'{c}, Veselin and Zemunik, Tatijana and Barbali\'{c}, Maja and Novak, Ivana and Pe\v{s}uti\'{c} Pisac, Valdi and Punda, Ante and Boraska Perica, Vesna}, year = {2020}, pages = {11}, DOI = {10.1038/s41598-020-58457-x.}, chapter = {1754}, keywords = {Hashimoto’s thyroiditis, AATF, SMARCA2, genome-wide association study (GWAS)}, journal = {Scientific reports}, doi = {10.1038/s41598-020-58457-x.}, volume = {10}, issn = {2045-2322}, title = {AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients}, keyword = {Hashimoto’s thyroiditis, AATF, SMARCA2, genome-wide association study (GWAS)}, chapternumber = {1754} }
@article{article, author = {Br\v{c}i\'{c}, Luka and Bari\'{c}, Ana and Benzon, Benjamin and Brekalo, Marko and Gra\v{c}an, Sanda and Kali\v{c}anin, Dean and \v{S}krabi\'{c}, Veselin and Zemunik, Tatijana and Barbali\'{c}, Maja and Novak, Ivana and Pe\v{s}uti\'{c} Pisac, Valdi and Punda, Ante and Boraska Perica, Vesna}, year = {2020}, pages = {11}, DOI = {10.1038/s41598-020-58457-x.}, chapter = {1754}, keywords = {Hashimoto’s thyroiditis, AATF, SMARCA2, genome-wide association study (GWAS)}, journal = {Scientific reports}, doi = {10.1038/s41598-020-58457-x.}, volume = {10}, issn = {2045-2322}, title = {AATF and SMARCA2 are associated with thyroid volume in Hashimoto's thyroiditis patients}, keyword = {Hashimoto’s thyroiditis, AATF, SMARCA2, genome-wide association study (GWAS)}, chapternumber = {1754} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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