engleski

Cytomegalovirus Infection: Mouse Model

This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay.

mice ; cytomegalovirus infections/virology ; disease models ; animal ; virus replication ; viral plaque assay/methods ; viral load ; salivary gland/virology ; mouse cytomegalovirus/isolation & purification ; herpesviridae infections/virology ; fibroblasts/virology ; MCMV

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