engleski

Characterization of anthocyanins in chokeberry and elderberry by using RP-HPLC method

Anthocyanins are polyphenolic compounds with many positive bioactivities, found in high amounts in fruits, especially in fruits like elderberries and chokeberries. The aim of this study was to develop and validate reversed phase high-performance liquid chromatography method (RP-HPLC) and to characterize anthocyanins in chokeberry and elderberry. A gradient was developed by using 0.5 % phosphoric acid and a 100 % acetonitril, an RP- HPLC method was validated by determining linearity, limit of detection (LOD), limit of quantification (LOQ), sensitivity, precision and accuracy of the method. Anthocyanins were extracted from chokeberry and elderberry by using ultrasonic assisted extraction and 0, 1% hydrochloric acid in methanol as a solvent. The identification of anthocyanins was carried out by comparing UV/Vis spectrum and retention times with authentic standards or speaking extracts with authentic standards. Anthocyanins were quantified by using developed RP-HPLC method. Three anthocyanins were identified and quantified in elderberry (cyanidin-3- sambubioside-5-glucoside, cyanidin-3- sambubioside, and cyanidin-3- glucoside, 3203, 10163, 3981 mg/kg of fresh fruit weight, respectively) and four in chokeberry (cyanidin- 3-galactoside, cyanidin-3-glucoside, cyanidin- 3-arabinoside, and cyanidin-3-xyloside, 8221, 474, 3773, 691 mg/kg of fresh fruit weight, respectively). The amounts of anthocyanins show that elderberry and chokeberry are rich sources of anthocyanins

validation ; identification ; RP-HPLC ; anthocyanins ; chokeberry ; elderberry ;

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