Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines (CROSBI ID 274634)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Logarušić, Marijan ; Slivac, Igor ; Radošević, Kristina ; Bagović, Martina ; Radojčić Redovniković, Ivana ; Gaurina Srček, Višnja
engleski
Hempseed protein hydrolysates’ effects on the proliferation and induced oxidative stress in normal and cancer cell lines
Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isolate. The HPHs were prepared by enzyme hydrolysis using three different proteases of microbial origin: Alcalase®, Neutrase® and Protamex®. The antioxidant activity of the obtained hydrolysates was determined by oxygen radical absorbance capacity (ORAC) assay, while the proliferative effects on normal (HaCaT) and cancer (HeLa) cells were determined by the CellTiter 96® AQueous One Solution Reagent (MTS) assay. HPHs showed dosedependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase® (HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on H2O2-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products.
Antioxidant activity ; Antiproliferative effects ; Hempseed protein hydrolysates ; ORAC value
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Podaci o izdanju
46
2019.
6079-6085
objavljeno
0301-4851
1573-4978
10.1007/s11033-019-05043-8