Granulysin‐mediated apoptosis of trophoblasts in blighted ovum and missed abortion (CROSBI ID 274569)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Gulic, Tamara ; Laskarin, Gordana ; Dominovic, Marin ; Glavan Gacanin, Lana ; Babarović, Emina ; Rubesa, Zeljka ; Haller, Herman ; Rukavina, Daniel
engleski
Granulysin‐mediated apoptosis of trophoblasts in blighted ovum and missed abortion
PROBLEM: Granulysin (GNLY) is a cytotoxic molecule mostly present in decidual natural killer (NK) cells. Blighted ovum (BO) and missed abortion (MA) represent the early pathological pregnancies with hindered development of the embryoblast or a dead embryo. We investigated the GNLY-mediated apoptotic mechanism potentially responsible for delayed termination of pregnancy. METHOD OF STUDY: We performed immunohistological and immunofluorescence labeling of decidual tissues (GNLY, Apaf-1, NF-κB). NKG2A expression was analyzed by flow cytometry and GNLY mRNA by RT- qPCR. RESULTS: The GNLY labeling intensity (H score) was lower in the nuclei of trophoblast cells in BO and MA. GNLY gene levels were inversely detected in BO and MA. A decreased decidual NK cell percentage was found in MA. NK cells from pathological pregnancies expressed lower NKG2A levels. The highest frequency of Apaf-1 was found in trophoblast cells of MA. NF-kB was highly expressed in decidual cells of BO. CONCLUSION: The reduced activation of GNLY-mediated killing might be implicated in the slower rejection of trophoblast cells in BO and MA. A decreased authentic decidual NK cell number could be responsible for low cytotoxicity against trophoblast cells in MA. In BO, trophoblast cells have a higher survival potential due to increased NF-kB expression.
Apaf-1 ; blighted ovum ; granulysin ; missed abortion ; NF-kB ; pregnancy
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Podaci o izdanju
80 (3)
2018.
1-11
objavljeno
1046-7408
1600-0897
10.1111/aji.12978