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Pregled bibliografske jedinice broj: 1045881

Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design


(Carbazymes) Česnik, Morana; Sudar, Martina; Hernández, Karel; Charnock, Simon; Vasić-Rački, Đurđa; Clapés, Pere; Findrik Blažević, Zvjezdana
Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design // Reaction Chemistry and Engineering, 5 (2020), 747-759 doi:10.1039/C9RE00453J (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1045881 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design

Autori
Česnik, Morana ; Sudar, Martina ; Hernández, Karel ; Charnock, Simon ; Vasić-Rački, Đurđa ; Clapés, Pere ; Findrik Blažević, Zvjezdana

Kolaboracija
Carbazymes

Izvornik
Reaction Chemistry and Engineering (2058-9883) 5 (2020); 747-759

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aldolase kinetics, transaminase kinetics, process optimisation, mathematical modelling, cascade reaction, L-homoserine

Sažetak
L-Homoserine is an important compound and a building block in chemical and pharmaceutical industry. The one-pot cascade synthesis of L- homoserine with substrate recycling catalysed by an aldolase and a transaminase was investigated in detail, with special interest in the reaction’s kinetics and process development. Complex relationships between the process variables were discovered and the kinetic and reactor model were developed. In addition, new chemical insights were developed through this model-driven process development, and for the first time a kinetic model was proposed to describe the YfaU-catalysed reaction. The modelling was crucial for a better understanding of the reaction system, as well as for process optimisation. Finding a proper strategy for process improvement by manipulating the reaction conditions, as well as developing process design, was imperative to achieve the process metrics necessary for scale-up. Using the optimized reaction conditions in the fed-batch reactor with a continuous formaldehyde supply, and with subsequent addition of pyruvate and L-alanine, 80.1 g L–1 of L-homoserine was obtained with a volume productivity of 3.2 g L–1 h–1 after 25 h. These results present a 100% increase in product concentration and approximately 18% increase in volume productivity in comparison with previously published work. This work shows the benefit of the synergistic approach of bio(chemical) knowledge and process model development working hand-in-hand which is crucial for stronger breakthrough of biocatalysis to chemical industry.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA


Projekti:
EK-H2020-635595 - Sustainable industrial processes based on a C-C bond-forming enzyme platform (CARBAZYMES) (Findrik Blažević, Zvjezdana, EK - H2020-LEIT-BIO-2014-1) ( POIROT)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

doi

Citiraj ovu publikaciju

(Carbazymes) Česnik, Morana; Sudar, Martina; Hernández, Karel; Charnock, Simon; Vasić-Rački, Đurđa; Clapés, Pere; Findrik Blažević, Zvjezdana
Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design // Reaction Chemistry and Engineering, 5 (2020), 747-759 doi:10.1039/C9RE00453J (međunarodna recenzija, članak, znanstveni)
(Carbazymes) (Carbazymes) Česnik, M., Sudar, M., Hernández, K., Charnock, S., Vasić-Rački, Đ., Clapés, P. & Findrik Blažević, Z. (2020) Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design. Reaction Chemistry and Engineering, 5, 747-759 doi:10.1039/C9RE00453J.
@article{article, year = {2020}, pages = {747-759}, DOI = {10.1039/C9RE00453J}, keywords = {aldolase kinetics, transaminase kinetics, process optimisation, mathematical modelling, cascade reaction, L-homoserine}, journal = {Reaction Chemistry and Engineering}, doi = {10.1039/C9RE00453J}, volume = {5}, issn = {2058-9883}, title = {Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design}, keyword = {aldolase kinetics, transaminase kinetics, process optimisation, mathematical modelling, cascade reaction, L-homoserine} }
@article{article, year = {2020}, pages = {747-759}, DOI = {10.1039/C9RE00453J}, keywords = {aldolase kinetics, transaminase kinetics, process optimisation, mathematical modelling, cascade reaction, L-homoserine}, journal = {Reaction Chemistry and Engineering}, doi = {10.1039/C9RE00453J}, volume = {5}, issn = {2058-9883}, title = {Cascade enzymatic synthesis of L-homoserine – mathematical modelling as a tool for process optimisation and design}, keyword = {aldolase kinetics, transaminase kinetics, process optimisation, mathematical modelling, cascade reaction, L-homoserine} }

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