engleski

Multi-targeted screening of phytoestrogens in food, raw material, and dietary supplements by liquid chromatography with tandem mass spectrometry

This work was carried out to develop a high- performance liquid chromatography tandem mass spectrometry method for simultaneous determination of 11 marker compounds in phytoestrogen-rich food and dietary supplements (raw materials and formulated products) prepared from various sources: soy, red clover, chastetree, hop, and flax. The method was fully validated and showed detection limits varying from 0.2 to 1.5 μg/mL, average recoveries ranging from 95 to 105%, and relative standard deviation values lower than 4.98%. Evidently lower amounts of phytoestrogens were found in food samples than in the dietary supplements. Calculations for the actual daily dose of total phytoestrogens ranged from 0.01 to 6.61 mg among the different products. All derived total phytoestrogen amounts turned out to be below the total content stated by manufacturers (less than 63% of the claimed specifications). Moreover, two samples did not contained phytoestrogens at all. The most eye-catching batch-tobatch deviations were represented by chasteberry-based products (relative standard deviation higher than 9.8%).

phytoestrogens ; multi-target analysis ; liquid chromatography tandem mass spectrometry ; dietary supplements

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