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Protection of lipid membranes during oxidative stress using flavonoid embedded silica nanoparticles

Mesoporous silica nanoparticles were synthesized and stabilized using polyethylene glycol. Characterization was carried out using various methods, including XRD, FE-SEM and AFM. Specific surface was determined using BET analysis and electrokinetic measurements were performed in order to elucidate properties of MSNs in liquid media. MSNs were loaded with structurally different flavonoids. It was shown that it is possible to efficiently load and release flavonoids with different physicochemical and structural properties. Interaction with model membranes was investigated using AFM and the protective role of flavonoid was monitored before and after induced lipid peroxidation. Lipid peroxidation was induced by addition of hydrogen peroxide. The nanomechanical properties of DOPC membranes after lipid peroxidation confirmed membrane damage, which was attenuated when using flavonoid loaded nanoparticles. By applying combination of experimental techniques, this work generated detailed knowledge about the effects of flavonoid loaded MSNs on the elasticity of model membranes, especially under oxidative stress conditions.

flavonoids ; silica nanoparticles ; membrane protection ; lipid peroxidation

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