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Pregled bibliografske jedinice broj: 1043379

Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti-Kasha Photochemistry


Zlatić, Katarina; Antol, Ivana; Uzelac, Lidija; Mikecin Dražić, Ana-Matea; Kralj, Marijeta; Bohne, Cornelia; Basarić, Nikola
Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti-Kasha Photochemistry // ACS Applied Materials & Interfaces, 12 (2020), 1; 347-351 doi:10.1021/acsami.9b19472 (međunarodna recenzija, članak, znanstveni)


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Naslov
Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti-Kasha Photochemistry
(Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti- Kasha Photochemistry)

Autori
Zlatić, Katarina ; Antol, Ivana ; Uzelac, Lidija ; Mikecin Dražić, Ana-Matea ; Kralj, Marijeta ; Bohne, Cornelia ; Basarić, Nikola

Izvornik
ACS Applied Materials & Interfaces (1944-8244) 12 (2020), 1; 347-351

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
BODIPY ; Fluorescence ; Proteins ; Photochemistry ; Quinone methide

Sažetak
A novel approach for the photolabeling of proteins by a BODIPY fluorophore is reported that is based on an anti-Kasha photochemi-cal reaction from an upper singlet excited state (Sn) leading to the deamination of the BODIPY quinone methide precursor. On the other hand, the high photochemical stability of the dye upon excita-tion by visible light to S1 allows for the selective fluorescence detection from the dye or dye-protein adduct, without concomitant bleaching or hydrolysis of the protein-dye adduct. Therefore, pho-tolabeling and fluorescence monitoring can be uncoupled by using different excitation wavelengths. Combined theoretical and experi-mental studies by preparative irradiations, fluorescence and laser flash photolysis fully disclose the photophysical properties of the dye and its anti- Kasha photochemical reactivity. The application of the dye was demonstrated on photolabeling of bovine serum albu-min.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-5660 - Mulitidisciplinarni pristup otkriću lijekova s ciljanim djelovanjem na matične stanice tumora – uloga transporta kalija (MultiCaST) (Kralj, Marijeta, HRZZ - 2013-11) ( POIROT)
HRZZ-IP-2014-09-6312 - Supramolekulska kontrola fotokemijskih reakcija eliminacije (SupraPhotoE) (Basarić, Nikola, HRZZ - 2014-09) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Zlatić, Katarina; Antol, Ivana; Uzelac, Lidija; Mikecin Dražić, Ana-Matea; Kralj, Marijeta; Bohne, Cornelia; Basarić, Nikola
Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti-Kasha Photochemistry // ACS Applied Materials & Interfaces, 12 (2020), 1; 347-351 doi:10.1021/acsami.9b19472 (međunarodna recenzija, članak, znanstveni)
Zlatić, K., Antol, I., Uzelac, L., Mikecin Dražić, A., Kralj, M., Bohne, C. & Basarić, N. (2020) Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti-Kasha Photochemistry. ACS Applied Materials & Interfaces, 12 (1), 347-351 doi:10.1021/acsami.9b19472.
@article{article, year = {2020}, pages = {347-351}, DOI = {10.1021/acsami.9b19472}, keywords = {BODIPY, Fluorescence, Proteins, Photochemistry, Quinone methide}, journal = {ACS Applied Materials and Interfaces}, doi = {10.1021/acsami.9b19472}, volume = {12}, number = {1}, issn = {1944-8244}, title = {Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti-Kasha Photochemistry}, keyword = {BODIPY, Fluorescence, Proteins, Photochemistry, Quinone methide} }
@article{article, year = {2020}, pages = {347-351}, DOI = {10.1021/acsami.9b19472}, keywords = {BODIPY, Fluorescence, Proteins, Photochemistry, Quinone methide}, journal = {ACS Applied Materials and Interfaces}, doi = {10.1021/acsami.9b19472}, volume = {12}, number = {1}, issn = {1944-8244}, title = {Labeling of Proteins by BODIPY-Quinone Methides utilizing Anti- Kasha Photochemistry}, keyword = {BODIPY, Fluorescence, Proteins, Photochemistry, Quinone methide} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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