engleski

NMR study on interaction of silver nanoparticles with biothiols

Introduction of nanoparticles (NPs) to biological environments results in the formation of the so-called biomolecular corona a layer of adsorbed biomolecules on the surface of NPs. The composition and nature of corona and its impact on pharmacodynamics and pharmacokinetics of thusly-modied NPs is a major question in nanomedicine. In this study, we employed nuclear magnetic resonance (NMR) to evaluate interaction of silver NPs with glutathione (GSH), an important biothiol. The selected silver NPs were functionalized with three distinct coatings - non-ionic polyvinylpyrrolidone (PVP), positively charged poly-Llysine (PLL), and negatively charged sodium bis(2- ethylhexyl)sulfosuccinate (AOT). The experiments involved exposing diferent silver NPs to GSH, analysis of size and surface charge by light scattering technique and tracking the change in NMR spectra of GSH.

NMR ; protein corona ; silver nanoparticles

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