engleski

The effect of elevated temperature on oxidative stress in Arabidopsis thaliana with modified BPM genes expression

BPM1 protein from Arabidopsis thaliana is a part of a protein family that contains MATH (Meprin and TRAF Homology) and BTB/POZ (Bric-a-Brac, Tramtrack, Broad Complex) domains. Most proteins of the MATH-BTB family participate in the ubiquitin-dependent protein degradation in which, within the cullin3 dependent E3 ligase, selectively target specific proteins for ubiquitination and degradation on proteasomes. It is well known that BPM1, apart from regulation of developmental processes, plays an important role in phenotypic and physiological adaptability and the ability of plants to survive in a changing environment. Preliminary research has shown that BPM1 is accumulated at elevated temperature and some of the proteins involved in oxidative stress are BPM1 protein partners. Therefore, the aim of this study was to investigate the effect of elevated temperature on oxidative stress in Arabidopsis. Seedlings with overexpressed (OEBPM1) and suppressed (amiR-bpm) BPM1 gene, along with the wild type were exposed to a temperature of 37 °C for six hours. Plant material was collected immediately after treatment and after 24-hour recovery at the temperature of cultivation (24 °C). Content of hydrogen peroxide (H2O2) and level of lipid peroxidation, as well as activity of catalase (CAT), superoxide dismutase (SOD), ascorbate (APX) and guaiacol (GPOX) peroxidases were measured. After treatment at 37 °C, a change in the content of H2O2 and the activity of the APX and SOD in the wild type were observed. Compared to the wild type, OEBPM1 and amiR-bpm lines have a different dynamics in the above-mentioned parameters. The obtained results indicate dependence of certain biochemical parameters of oxidative stress upon BPM proteins.

amiR-bpm, antioxidative enzymes, H2O2, OEBPM1, thermotolerance

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