Environmental DNA in subterranean biology update: from “Where?” to “How many?” (CROSBI ID 686016)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Gorički, Špela ; Presetnik, Primož ; Prosenc- Zmrzljak, Uršula ; Blatnik, Matej ; Gredar, Tajda ; Kogovšek, Blaž ; Koit, Oliver ; Strah, Sara ; Bilandžija, Helena ; Jalžić, Branko ; Aljančič, Gregor ; Košir, Rok ; Mayaud, Cyril ; Štebih, Dejan ; Hudoklin, Andrej ; Jeffery, William
engleski
Environmental DNA in subterranean biology update: from “Where?” to “How many?”
Recent records of Proteus anguinus outside its historically known range (Gorički et al. 2017), discovered through detection of its DNA dissolved in groundwater (environmental DNA or eDNA), mark the beginning of a new era in the study and conservation of cryptic subterranean biodiversity. An upgraded technology, droplet digital PCR (ddPCR), initially developed for studies of gene expression, detection of genetically modified organisms and in medical diagnostics, is being tested for improved detection of the much smaller and rare stygobiont, the cave clam Congeria jalzici. In parallel to eDNA assay development for various stygobiotic species of the Dinaric Karst, a groundwater-sample library is being created. The samples will be available for future analysis of their species composition and will also serve as a source of information on any changes in species distribution over time. In another line of eDNA research, the utility of ddPCR for direct quantification of eDNA molecules in groundwater is being explored by using the large, accessible and well- characterized (Zakšek and Trontelj 2017) natural Proteus population in the Planina Cave (Slovenia) as a model. The eDNA methodology may in the future be applied in estimation and monitoring of Proteus population sizes without having to see, mark or otherwise disturb the animals themselves.
cave species ; threatened ; conservation ; Dinaric karst ; distrubution range
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
e29968
2018.
objavljeno
10.3897/aca.1.e29968
Podaci o matičnoj publikaciji
ARPHA Conference Abstracts (ACA)
2603-3925
Podaci o skupu
24th International Conference on Subterranean Biology
poster
20.08.2018-24.08.2018
Aveiro, Portugal