engleski

Triacylglycerols composition of oil in OS soybean cultivars

Oil content in grain and triacylglycerol composition of several Craotian soybean cultivars (Glycine max (L.) Merrill) was investigated. Trials were conducted at two localities during three years and involved 7 soybean cultivars. All investigated cultivars were created in soybean breeding programme at the Agricultural Insitute Osijek in Croatia. Oil content in grain was determined by NMR method. Triacylglycerols were analyzed by reversed phase high performance liquid chromatography with refractive infex detector and identified by comparing their retention time to standards. Oil content was similar in soybean cultivars within year and localities but varied significantly among years. Chromatograms of every injected sample showed 15 individual triacylglycerol peaks, and the main components are trilinolein, dilinoleoolein and dilinoleopalmitin. A small difference of triacylglycerol composition was noticed among soybean cultivars whereas larger one was noticed among the investigated years. The obrained results will be able to use in further breeding work for soybean cultivars grain quality improvement.

soybean; oil content; soybean oil; liquid chromatography; triacylglycerols

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