engleski

The kinetics of bovine serum albumine adsorption on calcium phosphate and TiO2 nanoparticles or nanotubes nanocomposites

One of the largest health issue in modern society is increased frequency of hard tissues chronic diseases. Often the only treatment of such diseases is implementation with the aim to regenerate damaged or diseased tissue. Considering that the main inorganic component of hard tissue is biological apatite, form of calcium phosphate (CaP), composite materials based on calcium phosphate and different inorganic nanomaterials attracts attentions as possible new and innovative implant materials. Among different nanomaterials, TiO2 nanomaterials stand out because they can improve mechanical properties of CaP. Albumine is one of three soluble proteins which adsorbs on the surface of implant materialsright after its implantation and affect their behavior in vivo. But, its role in formation of calcium phosphates on titanium implants is still not clarified. Therefore, the aim of this study is to investigate the adsorption of bovine serum albumin (BSA) on nanocomposites of calcium phosphate (CaP) and TiO2 nanoparticles (CaP/TiNP) or nanotubes (CaP/TiNT). In order to do that, the kinetics of BSA adsorption kinetics of BSA adsorption on TiO2 nanoparticles (TiNP), titanate nanotubes (TiNT), calcium deficient hydoxiapatite (CaDHA) obtained in control system, CaP/TiNP and CaP/TiNT was measured in situ on UV/VIS scanning spectrophotomer. Samples obtained after adsorption were filtered and given for further analysis which included Fourier- transform infrared apectroscopy (FTIR), powder x-ray diffraction (XRD) and scanning electron microscopy (SEM). The results of kinetics measurements were modeled using kinetics models of pseudo-first, pseudo-second rate and interparticle diffusion model. From the results of various kinetic models, the adsorption kinetics were found to follow pseudo-second- order rate kinetic model for CaP/TiNT nanocomposites, respectively pseudo-second- order rate and interparticle diffusion model for CaP/TiNP nanocomposites. Results obtained from FTIR, XRD and SEM analysis showed that albumin did not influence the composition and solid phase morphology

Calcium Phosphates ; TiO2 Nanomaterials ; Bovine Serum Albumin

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